Cyclin C anticorps (AA 290-303)

N° du produit ABIN967636

Détail du produit anti-Cyclin C anticorps

Antigène: Cyclin C (CCNC)

Épitope: AA 290-303

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin C est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.

Immunogène: Human Cyclin C polypeptide fragment aa. 290-303

Information d'application

Indications d'application: The antibodies may be used for western blot analysis (1:1000) and immunoprecipitation (1:100). Cyclin C has a molecular weight of 36 kD, however a 45 kD protein may also be observed in some cell types using the antiserum. The identity of this protein has not been determined, but may represent an alternative splice product of the cyclin C gene, as has been identified in other species. HeLa human cervical carcinoma (ATCC CCL 2) and NIH 3T3 mouse fibroblasts (ATCC CRL 1658) are suggested as a positive controls for these applications.

Commentaires:

Related Products: ABIN967390, ABIN968538

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Cyclin C (ABIN967636)

Liu, Ueda, Miyazaki, Tanaka, Mine, Tanaka, Taniguchi, Yamamura, Minami: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." dans: Molecular and cellular biology, Vol. 18, Issue 6, pp. 3445-54, (1998) (PubMed).

Bates, Rowan, Vousden: "Characterisation of human cyclin G1 and G2: DNA damage inducible genes." dans: Oncogene, Vol. 13, Issue 5, pp. 1103-9, (1996) (PubMed).

Li, Lahti, Kidd: "Alternatively spliced cyclin C mRNA is widely expressed, cell cycle regulated, and encodes a truncated cyclin box." dans: Oncogene, Vol. 13, Issue 4, pp. 705-12, (1996) (PubMed).

Demetrick, Matsumoto, Hannon, Okamoto, Xiong, Zhang, Beach: "Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3)." dans: Cytogenetics and cell genetics, Vol. 69, Issue 3-4, pp. 190-2, (1995) (PubMed).

Tassan, Jaquenoud, Léopold, Schultz, Nigg: "Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8871-5, (1995) (PubMed).

Sherr: "Mammalian G1 cyclins." dans: Cell, Vol. 73, Issue 6, pp. 1059-65, (1993) (PubMed).

Détails sur Cyclin C

Antigène: Cyclin C (CCNC)

Autre désignation: Cyclin C (CCNC Produits)

Synonymes: anticorps CG1C_DROME, anticorps CG7281, anticorps CYCC, anticorps Cln3, anticorps Cyc-C, anticorps DmcycC, anticorps Dmel\\CG7281, anticorps SRB11, anticorps cycC, anticorps cyclinC, anticorps dTRAP33, anticorps fj11d06, anticorps wu:fj11d06, anticorps zgc:73078, anticorps Cyclin-C, anticorps CycC, anticorps AI451004, anticorps AU020987, anticorps CG1C, anticorps Cyclin C, anticorps cyclin C, anticorps cyclin C L homeolog, anticorps cyclin-C, anticorps CycC, anticorps ccnc, anticorps CCNC, anticorps Ccnc, anticorps ccnc.L, anticorps LOC5577815

Sujet: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate progression of the cell cycle. More recently, cyclins are being shown to have additional functions not restricted to cell cycle regulation. Thus, cyclins have been placed into functional groups as follows: Group 1 cyclins (A, B, D1, D2, D3, E and F) function primarily in cell cycle reguation, Group 2 cyclins (C and H) also play a role in transcriptional regulation, Group 3 cyclins (G1, G2 and I) may play a role distinct from either cell cycle or transcriptional regulation. Cyclin C has been identified in both human and chicken species. Like many cyclins, the expression of cyclin C oscillates throughout the cell cycle, with a peak observed during G1 phase. Cyclin C may play a dual role within the cell in its ability to regulate both cell cycle progression as well as gene transcription. Cyclin C associates with Cdk8, forming a complex which can induce gene transcription of Cdc2 (Cdk1). Because Cdc2 kinase is important for cell entry into mitosis, cyclin C’s ability to regulate cell cycle progression may be attributed, in part, to modulation of Cdc2 protein expression.6 Cyclin C has a predicted molecular weight of 36 kD. The polyclonal antiserum reacts with human and mouse cyclin C. A polypeptide fragment containing amino acids 290-303 (QGPNGSQNSSYSQS) at the C-terminus of human cyclin C, with the addition of a C-terminal cysteine (C) residue to facilitate conjugation to KLH, was used as immunogen.

Poids moléculaire: 36 kDa

Pathways: Cycle Cellulaire, Regulation of Lipid Metabolism by PPARalpha