MCL-1 anticorps (C-Term)
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- Antigène Voir toutes MCL-1 (MCL1) Anticorps
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
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Épitope
- C-Term
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MCL-1 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Marque
- BD Pharmingen™
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain
- Clone
- 22-Mcl
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MCL1 Anticorps primaire
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- Indications d'application
- The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).
- Commentaires
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Related Products: ABIN968586, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4° C.
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BCL-2 family members and the mitochondria in apoptosis." dans: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).
: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." dans: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, (1999) (PubMed).
: "The Bcl-2 protein family: arbiters of cell survival." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, (1998) (PubMed).
: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." dans: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, (1995) (PubMed).
: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, (1993) (PubMed).
: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." dans: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).
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BCL-2 family members and the mitochondria in apoptosis." dans: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).
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- Antigène
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
- Autre désignation
- Mcl-1 (MCL1 Produits)
- Synonymes
- anticorps BCL2L3, anticorps EAT, anticorps MCL1-ES, anticorps MCL1L, anticorps MCL1S, anticorps Mcl-1, anticorps TM, anticorps bcl2-L-3, anticorps mcl1/EAT, anticorps AW556805, anticorps mcl-1, anticorps fb72f02, anticorps wu:fb70a08, anticorps wu:fb72f02, anticorps zfMLP1, anticorps zfMcl-1a, anticorps zgc:109835, anticorps zgc:109763, anticorps E3, anticorps E3-2, anticorps RNE3, anticorps RNU107, anticorps SNORA63A, anticorps elF-4AII, anticorps MCL1, BCL2 family apoptosis regulator, anticorps myeloid cell leukemia sequence 1, anticorps MCL1, BCL2 family apoptosis regulator a, anticorps MCL1, BCL2 family apoptosis regulator b, anticorps small nucleolar RNA, H/ACA box 63, anticorps MCL1, anticorps Mcl1, anticorps mcl1a, anticorps mcl1b, anticorps SNORA63
- Sujet
- The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.
- Poids moléculaire
- 40 kDa
- Pathways
- Signalisation MAPK
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