CSK anticorps (AA 1-156)

N° du produit ABIN967730

Détail du produit anti-CSK anticorps

Antigène: CSK (C-Src tyrosine Kinase (CSK))

Épitope: AA 1-156

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CSK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Souris, Humain, Chien, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Csk aa. 1-156

Clone: 52-Csk

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968555, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CSK (ABIN967730)

Bénistant, Bourgaux, Chapuis, Mottet, Roche, Bali: "The COOH-terminal Src kinase Csk is a tumor antigen in human carcinoma." dans: Cancer research, Vol. 61, Issue 4, pp. 1415-20, (2001) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." dans: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Wan, Bence, Hata, Kurosaki, Veillette, Huang: "Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling." dans: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17209-15, (1997) (PubMed).

Bergman, Mustelin, Oetken, Partanen, Flint, Amrein, Autero, Burn, Alitalo: "The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity." dans: The EMBO journal, Vol. 11, Issue 8, pp. 2919-24, (1992) (PubMed).

Okada, Nada, Yamanashi, Yamamoto, Nakagawa: "CSK: a protein-tyrosine kinase involved in regulation of src family kinases." dans: The Journal of biological chemistry, Vol. 266, Issue 36, pp. 24249-52, (1992) (PubMed).

Détails sur CSK

Antigène: CSK (C-Src tyrosine Kinase (CSK))

Autre désignation: Csk (CSK Produits)

Synonymes: anticorps AW212630, anticorps C-terminal Src kinase, anticorps c-src tyrosine kinase, anticorps CSK, non-receptor tyrosine kinase, anticorps CSK, anticorps Csk

Sujet: The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60[c-src], p59[fyn], p53/56[lyn], p56[lck], and p62[yes]. This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59[fyn] and p53/56[lyn]. These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.

Poids moléculaire: 50 kDa

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events