FAK anticorps (AA 354-533)
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- Antigène Voir toutes FAK (PTK2) Anticorps
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
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Épitope
- AA 354-533
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Reactivité
- Humain, Souris, Rat, Poulet, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp FAK est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
- Réactivité croisée
- Humain, Souris, Rat (Rattus), Chien
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Chicken FAK aa. 354-533
- Clone
- 77-FAK
- Isotype
- IgG1
- Top Product
- Discover our top product PTK2 Anticorps primaire
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- Indications d'application
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - Commentaires
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Related Products: ABIN967389, ABIN968533
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store undiluted at -20°C.
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Nuclear compartmentalization of FAK and FRNK in cardiac myocytes." dans: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2509-15, (2006) (PubMed).
: "Nuclear import of N-terminal FAK by activation of the FcepsilonRI receptor in RBL-2H3 cells." dans: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 39-45, (2004) (PubMed).
: "PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration." dans: The Journal of cell biology, Vol. 160, Issue 1, pp. 137-46, (2003) (PubMed).
: "Insulin-like growth factor I prevents mannitol-induced degradation of focal adhesion kinase and Akt." dans: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 27393-400, (2002) (PubMed).
: "Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy." dans: Molecular biology of the cell, Vol. 12, Issue 8, pp. 2290-307, (2001) (PubMed).
: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." dans: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, (1999) (PubMed).
: "Outside-in signaling in the chondrocyte. Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex." dans: The Journal of clinical investigation, Vol. 100, Issue 7, pp. 1789-96, (1997) (PubMed).
: "
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Nuclear compartmentalization of FAK and FRNK in cardiac myocytes." dans: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2509-15, (2006) (PubMed).
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- Antigène
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- Autre désignation
- FAK (PTK2 Produits)
- Synonymes
- anticorps FADK, anticorps FAK, anticorps FAK1, anticorps FRNK, anticorps PPP1R71, anticorps p125FAK, anticorps pp125FAK, anticorps PTK2, anticorps Fadk, anticorps mKIAA4203, anticorps fak, anticorps fak1a, anticorps ptk2, anticorps ptk2a, anticorps wu:fa18c04, anticorps zgc:66114, anticorps CG10023, anticorps CT28129, anticorps DFAK, anticorps DFak, anticorps DFak56, anticorps Dfak, anticorps Dfak56, anticorps DmFAK, anticorps Dmel\\CG10023, anticorps Fak56, anticorps Fak56D, anticorps fak56, anticorps protein tyrosine kinase 2, anticorps protein tyrosine kinase 2 L homeolog, anticorps PTK2 protein tyrosine kinase 2, anticorps protein tyrosine kinase 2ab, anticorps Focal adhesion kinase, anticorps PTK2, anticorps ptk2.L, anticorps Ptk2, anticorps ptk2ab, anticorps Fak
- Sujet
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Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the Focal Adhesion Targeting sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation and activation of FAK. This creates binding sites for SH2 domains of intracellular signaling molecules such as src, PI3 kinase, and Grb2. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions has led to substantial speculation about its function. Although FAK's precise role has not been elucidated, proposed possibilities include regulating cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival.
Synonyms: Focal Adhesion Kinase - Poids moléculaire
- 116-125 kDa
- Pathways
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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