NCK1 anticorps (AA 279-377)

N° du produit ABIN967743

Détail du produit anti-NCK1 anticorps

Antigène: NCK1 (NCK Adaptor Protein 1 (NCK1))

Épitope: AA 279-377

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Grenouille, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Boeuf (Vache), Poulet, Chien, Grenouille, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human NCK aa. 279-377

Clone: 108-NCK

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NCK1 (ABIN967743)

Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." dans: Cell, Vol. 103, Issue 6, pp. 945-56, (2001) (PubMed).

Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." dans: Oncogene, Vol. 20, Issue 30, pp. 4058-69, (2001) (PubMed).

Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." dans: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, (2001) (PubMed).

McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." dans: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, (2000) (PubMed).

Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." dans: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, (1996) (PubMed).

Détails sur NCK1

Antigène: NCK1 (NCK Adaptor Protein 1 (NCK1))

Autre désignation: NCK (NCK1 Produits)

Synonymes: anticorps NCK, anticorps NCKalpha, anticorps nck-1, anticorps NCK1, anticorps 6330586M15Rik, anticorps D230010O13Rik, anticorps Nck, anticorps Nck-1, anticorps Nck1, anticorps nck, anticorps nck1-a, anticorps nck1-b, anticorps nckalpha, anticorps NCK adaptor protein 1, anticorps non-catalytic region of tyrosine kinase adaptor protein 1, anticorps NCK adaptor protein 1b, anticorps NCK adaptor protein 1 L homeolog, anticorps NCK1, anticorps Nck1, anticorps nck1b, anticorps nck1.L

Sujet: NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 47 kDa

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals