PKC alpha anticorps (AA 270-427)

N° du produit ABIN967750

Détail du produit anti-PKC alpha anticorps

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Épitope: AA 270-427

Reactivité: Humain, Souris, Rat, Chien, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus), Souris, Poulet, Chien, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PKCalpha aa. 270-427

Clone: 3-PKCalpha

Isotype: IgG2b kappa

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PKC alpha (ABIN967750)

Sakagami, Katsumata, Hara, Tamaki, Watanabe, Harvey, Fukaya: "Distinct synaptic localization patterns of brefeldin A-resistant guanine nucleotide exchange factors BRAG2 and BRAG3 in the mouse retina." dans: The Journal of comparative neurology, Vol. 521, Issue 4, pp. 860-76, (2013) (PubMed).

Blom, Giove, Deshpande, Eldred: "Characterization of nitric oxide signaling pathways in the mouse retina." dans: The Journal of comparative neurology, Vol. 520, Issue 18, pp. 4204-17, (2013) (PubMed).

Besalduch, Tomàs, Santafé, Garcia, Tomàs, Lanuza: "Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse." dans: The Journal of comparative neurology, Vol. 518, Issue 2, pp. 211-28, (2009) (PubMed).

Fujino, Wu, Lin, Phillips, Nedivi: "cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival." dans: The Journal of comparative neurology, Vol. 507, Issue 5, pp. 1831-45, (2008) (PubMed).

Powner, Hodgkin, Wakelam: "Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells." dans: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1252-62, (2002) (PubMed).

Wagner, Fowler, Takemoto: "The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells." dans: Molecular vision, Vol. 8, pp. 394-406, (2002) (PubMed).

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." dans: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, (2001) (PubMed).

Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." dans: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, (1996) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." dans: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

Détails sur PKC alpha

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PKC alpha (PKCa Produits)

Synonymes: anticorps pkc-alpha, anticorps pkcalpha, anticorps im:7139045, anticorps si:dkey-8l19.1, anticorps LOC100219195, anticorps AAG6, anticorps PKC-alpha, anticorps PKCA, anticorps PRKACA, anticorps AI875142, anticorps Pkca, anticorps PKRCA, anticorps protein kinase C alpha, anticorps protein kinase C, alpha L homeolog, anticorps protein kinase C, alpha, anticorps PRKCA, anticorps prkca.L, anticorps prkca, anticorps Prkca

Sujet: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Overexpression of PKCalpha has been reported to lead to an enhanced growth rate and induces the phosphorylation of two cellular proteins of 52 kDa and 90 kDa. This antibody is routinely tested by western blot analysis.
This antibody has been reported to also crossreact with PKCbeta.
Synonyms: Protein Kinase Calpha

Poids moléculaire: 82 kDa

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling