Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

RAP2A anticorps (AA 1-183)

RAP2A Reactivité: Humain, Souris, Rat, Chien, Poulet, Grenouille WB, IF, IP, IHC (f) Hôte: Souris Monoclonal 12-Rap2 unconjugated
N° du produit ABIN967831
  • Antigène Voir toutes RAP2A Anticorps
    RAP2A (RAP2A, Member of RAS Oncogene Family (RAP2A))
    Épitope
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-183
    Reactivité
    • 10
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Chien, Poulet, Grenouille
    Hôte
    • 10
    • 1
    Souris
    Clonalité
    • 11
    Monoclonal
    Conjugué
    • 11
    Cet anticorp RAP2A est non-conjugé
    Application
    • 11
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
     Réactivité croisée
    Poulet, Chien, Grenouille, Souris, Rat (Rattus)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Rap2 aa. 1-183
    Clone
    12-Rap2
    Isotype
    IgG2a
    Top Product
    Discover our top product RAP2A Anticorps primaire
  • Commentaires

    Related Products: ABIN968533, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Ohba, Mochizuki, Matsuo, Yamashita, Nakaya, Hashimoto, Hamaguchi, Kurata, Nagashima, Matsuda: "Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade." dans: Molecular and cellular biology, Vol. 20, Issue 16, pp. 6074-83, (2000) (PubMed).

    Reedquist, Bos: "Costimulation through CD28 suppresses T cell receptor-dependent activation of the Ras-like small GTPase Rap1 in human T lymphocytes." dans: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4944-9, (1998) (PubMed).

    Janoueix-Lerosey, Polakis, Tavitian, de Gunzburg: "Regulation of the GTPase activity of the ras-related rap2 protein." dans: Biochemical and biophysical research communications, Vol. 189, Issue 1, pp. 455-64, (1992) (PubMed).

    Ohmstede, Farrell, Reep, Clemetson, Lapetina: "RAP2B: a RAS-related GTP-binding protein from platelets." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 17, pp. 6527-31, (1990) (PubMed).

    Pizon, Chardin, Lerosey, Olofsson, Tavitian: "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region." dans: Oncogene, Vol. 3, Issue 2, pp. 201-4, (1988) (PubMed).

  • Antigène
    RAP2A (RAP2A, Member of RAS Oncogene Family (RAP2A))
    Autre désignation
    Rap2 (RAP2A Produits)
    Synonymes
    anticorps krev, anticorps rap2, anticorps k-rev, anticorps rbbp-30, anticorps RAP2A, anticorps K-REV, anticorps KREV, anticorps RAP2, anticorps RbBP-30, anticorps 5830461H18Rik, anticorps RAP2A, member of RAS oncogene family, anticorps Rap2A GTPase, anticorps RAP2B, member of RAS oncogene family, anticorps RAS related protein 2a, anticorps RAP2A, anticorps Rap2a, anticorps rap2a, anticorps RAP2B
    Sujet
    Rap2 is a member of the Ras superfamily of low molecular weight GTP/GDP binding proteins. The Rap proteins are 50% homologous in sequence to p21ras. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Conversion between these two forms is regulated by Rap-GTPase activating protein (Rap-GAP) and a Rap-GDP dissociation stimulator (GDS). Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap2 proteins are about 60% identical to Rap1 proteins and Rap2A and Rap2B show 90% amino acid identity, differing mainly at the carboxy-terminus. Unlike Rap1, the Rap2 proteins cannot compete with Ras for interaction with Ras-GAP, nor are they substrates for PKA. It follows that the intrinsic GTPase activity of Rap2A is not stimulated by Ras-GAP, however a distinct activator (Rap-GAP) has been identified. Both Rap2 proteins show posttranslational modifications: Rap2B is geranylgeranylated and Rap2A is the first non-Ras member of the Ras superfamily observed to be farnesylated. This antibody is routinely tested by western blot analysis.
    Poids moléculaire
    21 kDa
Vous êtes ici:
Support technique