Synaptotagmin anticorps (AA 72-223)

N° du produit ABIN967965

Détail du produit anti-Synaptotagmin anticorps

Antigène: Synaptotagmin (SYT)

Épitope: AA 72-223

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Synaptotagmin est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée: Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Synaptotagmin

Clone: BC17

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 µg/ml

Buffer: Aqueous buffered solution containing BSA, glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Synaptotagmin (ABIN967965)

Liu, Fallon, Lashuel, Liu, Lansbury: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." dans: Cell, Vol. 111, Issue 2, pp. 209-18, (2002) (PubMed).

Ramalho-Santos, Moreno, Sutovsky, Chan, Hewitson, Wessel, Simerly, Schatten: "SNAREs in mammalian sperm: possible implications for fertilization." dans: Developmental biology, Vol. 223, Issue 1, pp. 54-69, (2000) (PubMed).

Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." dans: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, (1999) (PubMed).

Scheller: "Membrane trafficking in the presynaptic nerve terminal." dans: Neuron, Vol. 14, Issue 5, pp. 893-7, (1995) (PubMed).

Perin, Johnston, Ozcelik, Jahn, Francke, Südhof: "Structural and functional conservation of synaptotagmin (p65) in Drosophila and humans." dans: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 615-22, (1991) (PubMed).

Détails sur Synaptotagmin

Antigène: Synaptotagmin (SYT)

Autre désignation: Synaptotagmin (SYT Produits)

Synonymes: anticorps Syti, anticorps GB20036, anticorps DKFZP459P193, anticorps SYT1, anticorps SytI, anticorps SyNapTotagmin, anticorps synaptotagmin, anticorps synaptotagmin 1, anticorps snt-5, anticorps snt-6, anticorps snt-3, anticorps Syt, anticorps Syt1, anticorps SYT1

Sujet: Synaptotagmin (p65) is an abundant synaptic vesicle protein that contains a single transmembrane region and two copies of an internal repeat that is homologous to the regulatory region of Protein Kinase C. It appears that synaptotagmin has a regulatory role in the synaptic vesicle pathway, particularly in vesicle docking and/or fusion with the plasmalemma. A model has been proposed to explain docking, activation, and fusion of synaptic vesicles with donor membranes. This model suggests that VAMP/synaptobrevin and synaptotagmin (vSNARE) on the synaptic vesicle, and SNAP-25 and syntaxin (tSNAREs) on the plasma membrane, interact to form a 7S complex. Two additional soluble proteins, alphaSNAP and NSF, are later added to the 7S complex, accompanied by the loss of synaptotagmin. The resulting 20S complex contains syntaxin, SNAP-25, VAMP, alphaSNAP, and NSF. Genetic studies in several species demonstrate that mutation or deletion of synaptotagmin results in a large decrease in Ca2+ triggered transmitter release. Mammalian synapses that lack synaptotagmin show a selective decrease in a fast component of release, suggesting that synaptotagmin is the Ca2+ sensor triggering exocytosis.

Poids moléculaire: 65 kDa