CBL anticorps (AA 595-810)

N° du produit ABIN967971

Détail du produit anti-CBL anticorps

Antigène: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Épitope: AA 595-810

Reactivité: Humain, Souris, Rat, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CBL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human c-Cbl aa. 595-810

Clone: 17-c

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-CBL (ABIN967971)

Jiang, Huang, Marusyk, Sorkin: "Grb2 regulates internalization of EGF receptors through clathrin-coated pits." dans: Molecular biology of the cell, Vol. 14, Issue 3, pp. 858-70, (2003) (PubMed).

Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." dans: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, (2001) (PubMed).

Garcia, Miller: "Single-cell analyses reveal two defects in peptide-specific activation of naive T cells from aged mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3151-7, (2001) (PubMed).

Sanjay, Houghton, Neff, DiDomenico, Bardelay, Antoine, Levy, Gailit, Bowtell, Horne, Baron: "Cbl associates with Pyk2 and Src to regulate Src kinase activity, alpha(v)beta(3) integrin-mediated signaling, cell adhesion, and osteoclast motility." dans: The Journal of cell biology, Vol. 152, Issue 1, pp. 181-95, (2001) (PubMed).

Donovan, Wange, Langdon, Samelson: "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen receptor." dans: The Journal of biological chemistry, Vol. 269, Issue 37, pp. 22921-4, (1994) (PubMed).

Détails sur CBL

Antigène: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Autre désignation: c-Cbl (CBL Produits)

Synonymes: anticorps 4732447J05Rik, anticorps Cbl-2, anticorps c-Cbl, anticorps cbl, anticorps C-CBL, anticorps CBL2, anticorps FRA11B, anticorps NSLL, anticorps RNF55, anticorps c-cbl-like, anticorps RGD1561386, anticorps Casitas B-lineage lymphoma, anticorps Cbl proto-oncogene, anticorps Cbl, anticorps CBL

Sujet: Cbl was identified in the genome of a transforming retrovirus from a mouse pre-B cell lymphoma. The product of mammalian c-cbl, p120[cbl], is a widely expressed cytoplasmic protein with several distinctive domains including a RING finger motif and a large proline-rich domain. Although the normal cellular function of c-Cbl has not yet been determined, it associates with many well known signal transduction proteins. In lymphoid cells, c-Cbl is a substrate of TCR-activated protein tyrosine kinases. In addition, stimulation with EGF, PDGF, FGF, or NGF results in tyrosine-phosphorylation of c-Cbl in lymphoid and other cell types. It forms a signaling complex with Grb2 in T cells and is a SH3 and SH2 binding protein which associates with many other cytoplasmic signaling proteins including Btk, Src, fyn, lck, and the beta isoform of PI-3 kinase. Thus, c-Cbl may be an important adaptor protein in growth factor and T-cell receptor signaling pathways. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 120 kDa

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals