Dynactin 1 anticorps (AA 3-202)

N° du produit ABIN967996

Détail du produit anti-Dynactin 1 anticorps

Antigène: Dynactin 1 (DCTN1)

Épitope: AA 3-202

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dynactin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Réactivité croisée: Humain, Poulet, Chien, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat p150 [Glued] aa. 3-202

Clone: 1-p150Glued

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Dynactin 1 (ABIN967996)

Kurzchalia: "Anthrax toxin rafts into cells." dans: The Journal of cell biology, Vol. 160, Issue 3, pp. 295-6, (2003) (PubMed).

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." dans: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, (2002) (PubMed).

Aumais, Tunstead, McNeil, Schaar, McConnell, Lin, Clark, Yu-Lee: "NudC associates with Lis1 and the dynein motor at the leading pole of neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 24, pp. RC187, (2001) (PubMed).

Tai, Chuang, Sung: "Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport." dans: The Journal of cell biology, Vol. 153, Issue 7, pp. 1499-509, (2001) (PubMed).

King, Schroer: "Dynactin increases the processivity of the cytoplasmic dynein motor." dans: Nature cell biology, Vol. 2, Issue 1, pp. 20-4, (2000) (PubMed).

Détails sur Dynactin 1

Antigène: Dynactin 1 (DCTN1)

Autre désignation: p150 Glued (DCTN1 Produits)

Synonymes: anticorps DAP-150, anticorps DP-150, anticorps P135, anticorps Chip150, anticorps DCTN1, anticorps DKFZp469C1813, anticorps AL022633, anticorps Glued, anticorps p150, anticorps p150, anticorps CG9206, anticorps DYNA_DROME, anticorps Dmel\\CG9206, anticorps FCP-D, anticorps Glu, anticorps P150, anticorps P150[Glued], anticorps p150/Glued, anticorps p150/glued, anticorps p150[Glued], anticorps p150[glued], anticorps t1, anticorps Dynactin-1, anticorps glued, anticorps dynactin subunit 1, anticorps dynactin 1, anticorps Dynactin 1, p150 subunit, anticorps dynactin subunit 1 L homeolog, anticorps DCTN1, anticorps Dctn1, anticorps DCTN1-p150, anticorps dctn1.L

Sujet: P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 150 kDa

Pathways: M Phase, ER-Nucleus Signaling