alpha Adaptin anticorps (AA 38-215)
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- Antigène Voir toutes alpha Adaptin (AP2A1) Anticorps
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
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Épitope
- AA 38-215
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Reactivité
- Humain, Souris, Rat, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp alpha Adaptin est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Réactivité croisée
- Rat (Rattus), Humain, Chien
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Mouse Adaptin alpha [A] aa. 38-215
- Clone
- 8-Adaptin alpha
- Isotype
- IgG1
- Top Product
- Discover our top product AP2A1 Anticorps primaire
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- Commentaires
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Related Products: ABIN968545, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store undiluted at -20°C.
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Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif." dans: Traffic (Copenhagen, Denmark), Vol. 4, Issue 7, pp. 468-78, (2003) (PubMed).
: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." dans: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, (2002) (PubMed).
: "The AP2 binding site of synaptotagmin 1 is not an internalization signal but a regulator of endocytosis." dans: The Journal of cell biology, Vol. 154, Issue 4, pp. 857-66, (2001) (PubMed).
: "Numb is an endocytic protein." dans: The Journal of cell biology, Vol. 151, Issue 6, pp. 1345-52, (2001) (PubMed).
: "The tyrosine kinase ACK1 associates with clathrin-coated vesicles through a binding motif shared by arrestin and other adaptors." dans: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 18392-8, (2001) (PubMed).
: "Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins)." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 833-42, (1989) (PubMed).
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Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif." dans: Traffic (Copenhagen, Denmark), Vol. 4, Issue 7, pp. 468-78, (2003) (PubMed).
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- Antigène
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
- Autre désignation
- Adaptin alpha (AP2A1 Produits)
- Synonymes
- anticorps ADTAA, anticorps AP2-ALPHA, anticorps CLAPA1, anticorps adtaa, anticorps clapa1, anticorps ap2-alpha, anticorps AP2A1, anticorps Adtaa, anticorps adaptor related protein complex 2 alpha 1 subunit, anticorps adaptor-related protein complex 2, alpha 1 subunit, anticorps AP-2 complex subunit alpha-1, anticorps AP2A1, anticorps ap2a1, anticorps Ap2a1, anticorps EDI_093420, anticorps EDI_259990
- Sujet
- Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (alpha, gamma, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. In the AP-2 complex, Adaptin alpha is expressed in two very similar isoforms from two different genes. Adaptin alpha [A] (112kDa) is expressed primarily in brain, while Adaptin alpha [C] (105kDa) is expressed in brain, liver and other tissues. This antibody is routinely tested by western blot analysis.
- Poids moléculaire
- 112 kDa
- Pathways
- Signalisation Notch, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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