Gephyrin anticorps (AA 569-726)

N° du produit ABIN968051

Détail du produit anti-Gephyrin anticorps

Antigène: Gephyrin (GPHN)

Épitope: AA 569-726

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Gephyrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Poulet, Humain, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Gephyrin aa. 569-726

Clone: 45-Gephyrin

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968546, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Gephyrin (ABIN968051)

Kneussel, Haverkamp, Fuhrmann, Wang, Wässle, Olsen, Betz: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, (2000) (PubMed).

Feng, Tintrup, Kirsch, Nichol, Kuhse, Betz, Sanes: "Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity." dans: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1321-4, (1998) (PubMed).

Meyer, Kirsch, Betz, Langosch: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." dans: Neuron, Vol. 15, Issue 3, pp. 563-72, (1995) (PubMed).

Prior, Schmitt, Grenningloh, Pribilla, Multhaup, Beyreuther, Maulet, Werner, Langosch, Kirsch: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." dans: Neuron, Vol. 8, Issue 6, pp. 1161-70, (1992) (PubMed).

Détails sur Gephyrin

Antigène: Gephyrin (GPHN)

Autre désignation: Gephyrin (GPHN Produits)

Synonymes: anticorps 5730552E08Rik, anticorps AI662856, anticorps BC027112, anticorps C230040D23, anticorps GPH, anticorps GPHRYN, anticorps geph, anticorps Geph, anticorps GPHN, anticorps GEPH, anticorps HKPX1, anticorps gpgb1, anticorps DKFZp459M0825, anticorps gephyrin, anticorps gephyrin L homeolog, anticorps gephyrin a, anticorps Gphn, anticorps GPHN, anticorps gphn.L, anticorps gphna, anticorps PTRG_08978, anticorps VDBG_00775, anticorps Tsp_03043, anticorps gphn

Sujet: The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 93 kDa

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development