NEK2 anticorps (AA 244-444)

N° du produit ABIN968054

Détail du produit anti-NEK2 anticorps

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Épitope: AA 244-444

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Nek2 aa. 244-444

Clone: 20-Nek2

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NEK2 (ABIN968054)

Fry, Schultz, Bartek, Nigg: "Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans." dans: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12899-905, (1995) (PubMed).

Schultz, Fry, Sütterlin, Ried, Nigg: "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans." dans: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 625-35, (1994) (PubMed).

Détails sur NEK2

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Autre désignation: Nek2 (NEK2 Produits)

Sujet: Reversible protein phosphorylation is critical for progression through the cell cycle and mitosis. In Aspergillus nidulans, the nima gene (never in mitosis) encodes a protein kinase that is essential for mitosis. Three human genes (nek1, 2, and 3 [Nima-related kinase]) with significant homology to the A. nidulans nima have been reported. The nek2 gene encodes a protein of 445 amino acids and, like its fungal homolog, its expression is regulated throughout the cell cycle. In Hela cells, Nek2 activity and expression are low during M and G1 phases of the cell cycle. However, both parameters increase during S phase and mitosis. In addition, Nek2 phosphorylates protein substrates exclusively at serine and threonine residues. Thus, like its fungal homolog, Nek2 may be a crucial element in controlling the cell's entry into S phase and mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Nima Related Kinase 2

Poids moléculaire: 46 kDa

Pathways: M Phase