IQGAP1 anticorps (AA 1348-1490)

N° du produit ABIN968063

Détail du produit anti-IQGAP1 anticorps

Antigène: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Épitope: AA 1348-1490

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IQGAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus), Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human IQGAP1 aa. 1348-1490

Clone: 24-IQGAP1

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-IQGAP1 (ABIN968063)

Mbele, Deloulme, Gentil, Delphin, Ferro, Garin, Takahashi, Baudier: "The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes." dans: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49998-50007, (2002) (PubMed).

Ruiz-Velasco, Lanning, Williams: "The activation of Rac1 by M3 muscarinic acetylcholine receptors involves the translocation of Rac1 and IQGAP1 to cell junctions and changes in the composition of protein complexes containing Rac1, IQGAP1, and actin." dans: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33081-91, (2002) (PubMed).

Li, Wang, Chakladar, Bronson, Bernards: "Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1." dans: Molecular and cellular biology, Vol. 20, Issue 2, pp. 697-701, (2000) (PubMed).

Hart, Callow, Souza, Polakis: "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs." dans: The EMBO journal, Vol. 15, Issue 12, pp. 2997-3005, (1996) (PubMed).

Kuroda, Fukata, Kobayashi, Nakafuku, Nomura, Iwamatsu, Kaibuchi: "Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1." dans: The Journal of biological chemistry, Vol. 271, Issue 38, pp. 23363-7, (1996) (PubMed).

Détails sur IQGAP1

Antigène: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Autre désignation: IQGAP1 (IQGAP1 Produits)

Synonymes: anticorps HUMORFA01, anticorps SAR1, anticorps p195, anticorps AA682088, anticorps D7Ertd237e, anticorps D7Ertd257e, anticorps mKIAA0051, anticorps IQ motif containing GTPase activating protein 1, anticorps IQGAP1, anticorps Iqgap1

Sujet: GTPases (Ras, Rho, cdc42Hs, and rac) modulate various cellular functions such as cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis (GTPases). IQGAP1 is Ras-GAP related protein that contains a WW domain that binds poly-proline regions, a calponin-homology domain (CHD), an IQ domain of unconventional myosins, and a C-terminal GAP-related domain (GRD). IQGAP1 co-immunoprecipitates with cdc42 and calmodulin, and colocalizes with cdc42Hs at the leading edges of the plasma membrane and at the cell-cell junctions. IQGAP1 may interact with both cadherins and catenins leading to dissociation of these proteins from sites of cell adhesion. Thus, IQGAP1 may be a Rac1 and Cdc42 effector that negatively regulates cadherin-mediated cell junctions.

Poids moléculaire: 195 kDa

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2