SMAD2 anticorps (AA 27-172)

N° du produit ABIN968106

Détail du produit anti-SMAD2 anticorps

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Épitope: AA 27-172

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SMAD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MAD2 aa. 27-172

Clone: 48-MAD2

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-SMAD2 (ABIN968106)

Babu, Jeganathan, Baker, Wu, Kang-Decker, van Deursen: "Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation." dans: The Journal of cell biology, Vol. 160, Issue 3, pp. 341-53, (2003) (PubMed).

Iwanaga, Kasai, Kibler, Jeang: "Characterization of regions in hsMAD1 needed for binding hsMAD2. A polymorphic change in an hsMAD1 leucine zipper affects MAD1-MAD2 interaction and spindle checkpoint function." dans: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31005-13, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Chen, Waters, Salmon, Murray: "Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores." dans: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 242-6, (1996) (PubMed).

Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." dans: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, (1996) (PubMed).

Détails sur SMAD2

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Autre désignation: hsMAD2 (SMAD2 Produits)

Synonymes: anticorps JV18, anticorps JV18-1, anticorps MADH2, anticorps MADR2, anticorps hMAD-2, anticorps hSMAD2, anticorps jv18, anticorps Xmad2, anticorps madh2, anticorps madr2, anticorps XSmad2, anticorps hmad-2, anticorps hsmad2, anticorps jv18-1, anticorps smad-2, anticorps fj43c06, anticorps wu:fj43c06, anticorps 7120426M23Rik, anticorps Madh2, anticorps Madr2, anticorps Smad-2, anticorps mMad2, anticorps MAD homolog 2, anticorps Mothers against DPP homolog 2, anticorps SMAD 2, anticorps Smad2, anticorps SMAD family member 2, anticorps SMAD family member 2-Z, anticorps SMAD family member 2 S homeolog, anticorps SMAD2, anticorps SMAD2Z, anticorps smad2, anticorps smad2.S, anticorps Smad2

Sujet: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. The human hsMAD2 gene encodes a protein of 205 amino acids with a predicted molecular weight of 23.5 kDa. Binding of affinity purified polyclonal antibodies to the MAD2 protein prevents mitosis of HeLa cells. This indicates that, like its invertebrate relatives, MAD2 is necessary for mitosis. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2.
Synonyms: Mitotic Arrest Deficient-2

Poids moléculaire: 24 kDa

Pathways: Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus