PKN1 anticorps (AA 215-388)

N° du produit ABIN968110

Détail du produit anti-PKN1 anticorps

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Épitope: AA 215-388

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Chien, Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PRK1 aa. 215-388

Clone: 49-PRK1

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-PKN1 (ABIN968110)

Hughes, Larijani, Parker: "Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ." dans: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22974-9, (2002) (PubMed).

Flynn, Mellor, Casamassima, Parker: "Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase." dans: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11064-70, (2000) (PubMed).

Palmer, Ridden, Parker: "Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family." dans: European journal of biochemistry / FEBS, Vol. 227, Issue 1-2, pp. 344-51, (1995) (PubMed).

Mukai, Kitagawa, Shibata, Takanaga, Mori, Shimakawa, Miyahara, Hirao, Ono: "Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis." dans: Biochemical and biophysical research communications, Vol. 204, Issue 1, pp. 348-56, (1994) (PubMed).

Détails sur PKN1

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Autre désignation: PRK1 (PKN1 Produits)

Synonymes: anticorps DBK, anticorps PAK-1, anticorps PAK1, anticorps PKN, anticorps PKN-ALPHA, anticorps PRK1, anticorps PRKCL1, anticorps F730027O18Rik, anticorps Pkn, anticorps Prkcl1, anticorps Stk3, anticorps PKN1, anticorps 3-2, anticorps CG2049, anticorps CG2055, anticorps CT6637, anticorps CT6660, anticorps DPKN, anticorps Dmel\\CG2049, anticorps Dpkn, anticorps PRK2, anticorps Pk45C, anticorps Pk?3, anticorps dPKN, anticorps l(2)06736, anticorps l(2)45Ca, anticorps v(2)rG232, anticorps protein kinase N1, anticorps protein kinase N1b, anticorps Protein kinase N, anticorps PKN1, anticorps Pkn1, anticorps pkn1b, anticorps pkn1, anticorps Pkn

Sujet: Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.
Synonyms: PKN

Poids moléculaire: 120 kDa