MCM2 anticorps (AA 725-888)

N° du produit ABIN968122

Détail du produit anti-MCM2 anticorps

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Épitope: AA 725-888

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human BM28 aa. 725-888

Clone: 46-BM28

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-MCM2 (ABIN968122)

Cook, Park, Burke, Leone, DeGregori, Engel, Nevins: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, (2002) (PubMed).

Shreeram, Sparks, Lane, Blow: "Cell type-specific responses of human cells to inhibition of replication licensing." dans: Oncogene, Vol. 21, Issue 43, pp. 6624-32, (2002) (PubMed).

Ishimi, Ichinose, Omori, Sato, Kimura: "Binding of human minichromosome maintenance proteins with histone H3." dans: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, (1996) (PubMed).

Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." dans: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, (1995) (PubMed).

Todorov, Pepperkok, Philipova, Kearsey, Ansorge, Werner: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." dans: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, (1994) (PubMed).

Détails sur MCM2

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Autre désignation: BM28 (MCM2 Produits)

Synonymes: anticorps CG7538, anticorps DmMCM2, anticorps DmMcm2, anticorps DmeMCM2, anticorps Dmel\\CG7538, anticorps MCM2, anticorps MCM2-7, anticorps MCM2_DROME, anticorps McM2, anticorps PCR3, anticorps dMCM2, anticorps l(3)rL074, anticorps XMcm2, anticorps bm28, anticorps ccnl1, anticorps cdc19, anticorps cdcl1, anticorps mitotin, anticorps cb737, anticorps chunp6905, anticorps BM28, anticorps CCNL1, anticorps CDCL1, anticorps D3S3194, anticorps MITOTIN, anticorps MCM7, anticorps AA959861, anticorps AW476101, anticorps Mcmd2, anticorps mKIAA0030, anticorps Minichromosome maintenance 2, anticorps minichromosome maintenance complex component 2, anticorps minichromosome maintenance complex component 2 L homeolog, anticorps Mcm2, anticorps mcm2, anticorps MCM2, anticorps mcm2.L

Sujet: BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.

Poids moléculaire: 125 kDa

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA