MSH6 anticorps (AA 225-333)

N° du produit ABIN968248

Détail du produit anti-MSH6 anticorps

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Épitope: AA 225-333

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MSH6 aa. 225-333

Clone: 44-MSH6

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-MSH6 (ABIN968248)

Kariola, Otway, Lönnqvist, Raevaara, Macrae, Vos, Kohonen-Corish, Hofstra, Nyström-Lahti: "Two mismatch repair gene mutations found in a colon cancer patient--which one is pathogenic?" dans: Human genetics, Vol. 112, Issue 2, pp. 105-9, (2003) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Humbert, Hermine, Hernandez, Bouget, Selves, Laurent, Salles, Lautier: "Implication of protein kinase C in the regulation of DNA mismatch repair protein expression and function." dans: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18061-8, (2002) (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." dans: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, (2000) (PubMed).

Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." dans: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, (1995) (PubMed).

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6 (MSH6 Produits)

Synonymes: anticorps GTBP, anticorps GTMBP, anticorps HNPCC5, anticorps HSAP, anticorps p160, anticorps cb326, anticorps wu:fb36c12, anticorps LOC100227810, anticorps AU044881, anticorps AW550279, anticorps Gtmbp, anticorps mutS homolog 6, anticorps mutS homolog 6 (E. coli), anticorps DNA mismatch repair protein, anticorps MSH6, anticorps msh6, anticorps Msh6

Sujet: DNA mismatch repair in bacteria is carried out by the MutL, MutH, and MutS proteins. Initial binding of MutS to the mismatched DNA is followed by binding of the MutH endonuclease and MutL. Together these proteins form a complex that mediates excision repair. Mutations or deficiencies of any of these bacterial proteins results in a mutator phenotype that is characterized by genetic instability. MSH2, MSH3, and MSH6 are human homologs of MutS, while MLH1, PMS1, and PMS2 are homologs of MutL. As a heterodimer with MSH2, MSH6 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes single-base mispairs and insertion/deletion loops. Binding of this complex induces conformational changes in the DNA that lead to the binding of an MLH-PMS1 complex and excision repair. Mutations in the human genes are associated with hereditary nonpolyposis colon cancer (HNPCC), a common hereditary disease in humans. HNPCC is characterized by frequent microsatellite mutations that arise from somatic mutation due to a replication error (RER+) phenotype. This phenotype is analogous to the bacterial system and is directly linked to DNA mismatch repair deficiencies. This antibody is routinely tested by western blot analysis.
Synonyms: GTBP

Poids moléculaire: 160 kDa

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response