UNC13B anticorps (AA 621-834)

N° du produit ABIN968305

Détail du produit anti-UNC13B anticorps

Antigène: UNC13B (Unc-13 Homolog B (UNC13B))

Épitope: AA 621-834

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UNC13B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Munc13-1 aa. 621-834

Clone: 32-panMunc13

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-UNC13B (ABIN968305)

Betz, Ashery, Rickmann, Augustin, Neher, Südhof, Rettig, Brose: "Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release." dans: Neuron, Vol. 21, Issue 1, pp. 123-36, (1998) (PubMed).

Sakaguchi, Orita, Naito, Maeda, Igarashi, Sasaki, Takai: "A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13." dans: Biochemical and biophysical research communications, Vol. 248, Issue 3, pp. 846-51, (1998) (PubMed).

Orita, Naito, Sakaguchi, Maeda, Igarashi, Sasaki, Takai: "Physical and functional interactions of Doc2 and Munc13 in Ca2+-dependent exocytotic machinery." dans: The Journal of biological chemistry, Vol. 272, Issue 26, pp. 16081-4, (1997) (PubMed).

Brose, Hofmann, Hata, Südhof: "Mammalian homologues of Caenorhabditis elegans unc-13 gene define novel family of C2-domain proteins." dans: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25273-80, (1995) (PubMed).

Détails sur UNC13B

Antigène: UNC13B (Unc-13 Homolog B (UNC13B))

Autre désignation: Munc13 (Pan) (UNC13B Produits)

Synonymes: anticorps MUNC13, anticorps UNC13, anticorps Unc13h2, anticorps UNC13B, anticorps Munc13-2, anticorps Munc13-1, anticorps Unc13a, anticorps Unc13h1, anticorps unc-13 homolog B, anticorps unc-13 homolog B (C. elegans), anticorps UNC13B, anticorps Unc13b

Sujet: Release of neurotransmitters at the synaptic junction is a primary mechanism of neuronal communication and is regulated by the synaptic vesicle (SV) cycle. Several components of the SV cycle were initially characterized in C. elegans. One of those, unc-13, is involved in normal presynaptic function. Mammalian homologues of unc-13 are Munc13-1, 13-2, and 13-3. These neuron-specific proteins lack N-terminal homology, but are highly conserved in their C-terminal regions. Here they contain a phorbal ester binding C1-domain and two C2-domains with homology to the PKC Ca2+/phospholipid-binding domain. The most abundant isoform, Munc 13-1, is a presynaptic receptor with high affinities for phorbol ester and DAG. In response to phorbol ester, Munc13-1 associates with the plasma membrane and acts as a stimulation dependent enhancer of neurotransmitter release. In addition, Munc13-1 interacts with components of the SV cycle (syntaxin, SNAP25, synaptobrevin, Doc2) and with a brain specific isoform of beta-spectrin, a protein that interacts with the actin cytoskeleton. Thus, it is thought that Munc13 proteins function in signaling pathways that regulate the neuronal exocytic machinery.

Poids moléculaire: 196 kDa

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis