RAD50 anticorps (AA 672-786)

N° du produit ABIN968313

Détail du produit anti-RAD50 anticorps

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Épitope: AA 672-786

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD50 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human RAD50 aa. 672-786

Clone: 13-RAD50

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAD50 (ABIN968313)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Huber, Yang, Sarkisian, Master, Deng, Chodosh: "Impaired DNA damage response in cells expressing an exon 11-deleted murine Brca1 variant that localizes to nuclear foci." dans: Molecular and cellular biology, Vol. 21, Issue 12, pp. 4005-15, (2001) (PubMed).

Ohta, Nicolas, Furuse, Nabetani, Ogawa, Shibata: "Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 646-51, (1998) (PubMed).

Trujillo, Yuan, Lee, Sung: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." dans: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, (1998) (PubMed).

Dolganov, Maser, Novikov, Tosto, Chong, Bressan, Petrini: "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair." dans: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4832-41, (1996) (PubMed).

Détails sur RAD50

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Autre désignation: Rad50 (RAD50 Produits)

Synonymes: anticorps NBSLD, anticorps RAD502, anticorps hRad50, anticorps Mrell, anticorps Rad50l, anticorps 6339, anticorps CG6339, anticorps Dmel\\CG6339, anticorps Dmrad50, anticorps RAD50, anticorps Rad50, anticorps ATRAD50, anticorps DNA REPAIR-RECOMBINATION PROTEIN, anticorps F22D22.28, anticorps F22D22_28, anticorps SPAP4C9.01c, anticorps im:6906849, anticorps si:ch211-79h18.3, anticorps RAD50 double strand break repair protein, anticorps CG6339 gene product from transcript CG6339-RE, anticorps RAD50 homolog, double strand break repair protein L homeolog, anticorps rad50, anticorps DNA repair protein RAD50, anticorps RAD50 homolog, double strand break repair protein, anticorps DNA repair-recombination protein (RAD50), anticorps MRX complex DNA-binding subunit, anticorps DNA repair protein Rad50, anticorps RAD50 recombination protein, anticorps Rad50 DNA repair/recombination protein, anticorps DNA repair protein rad-50, anticorps RAD50, anticorps Rad50, anticorps rad50, anticorps rad50.L, anticorps LOC658288, anticorps LOC726642, anticorps rad-50

Sujet: DNA double-strand breaks (DSBs) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes, meiotic and miotic recombination. DNA DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. Originally identified in S. cerevisiae, Rad50 is one of a group of genes, designated as the Rad52 epistasis group, whose products mediate DSB repair. Many of these genes, including Rad50, are conserved in humans and are thought to have a similar function to their S. cerevisiae counterparts. In yeast, a multiprotein complex of Rad50, MRE11, and XRS2 has been implicated in the nucleocytic processing of DSBs. In humans, Rad50 and MRE11 complex with up to three additional proteins (95 kDa, 200 kDa, and 350 kDa). The 95 kDa species is thought to be human XRS2, although a separate report has identified it as Nibrin, the product of the gene mutated in Nijmegen breakage syndrome. The Rad50-MRE11-p95 complex possess endonuclease and 3' to 5' exonuclease activity. Thus, human Rad50 functions in a multiprotein complex to mediate the repair of DSBs in the human genome. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 154 kDa

Pathways: Réparation de l'ADN, Protein targeting to Nucleus