GS28 anticorps (AA 3-108)

N° du produit ABIN968404

Détail du produit anti-GS28 anticorps

Antigène: GS28 (GOSR1) (Golgi SNAP Receptor Complex Member 1 (GOSR1))

Épitope: AA 3-108

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GS28 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat GS28 aa. 3-108

Clone: 1-GS28

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-GS28 (ABIN968404)

Loh, Peter, Subramaniam, Hong: "Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus." dans: Journal of cell science, Vol. 118, Issue Pt 6, pp. 1209-22, (2005) (PubMed).

Hamza, Prohaska, Gitlin: "Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1215-20, (2003) (PubMed).

Muller, Shorter, Newman, Deinhardt, Sagiv, Elazar, Warren, Shima: "Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion." dans: The Journal of cell biology, Vol. 157, Issue 7, pp. 1161-73, (2002) (PubMed).

Lanoix, Ouwendijk, Stark, Szafer, Cassel, Dejgaard, Weiss, Nilsson: "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1." dans: The Journal of cell biology, Vol. 155, Issue 7, pp. 1199-212, (2001) (PubMed).

Subramaniam, Loh, Hong: "N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex." dans: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25441-4, (1997) (PubMed).

Subramaniam, Peter, Philp, Wong, Hong: "GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport." dans: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1161-3, (1996) (PubMed).

Détails sur GS28

Antigène: GS28 (GOSR1) (Golgi SNAP Receptor Complex Member 1 (GOSR1))

Autre désignation: GS28 (GOSR1 Produits)

Synonymes: anticorps CG7700, anticorps Dmel\\CG7700, anticorps FSG28, anticorps GOS28, anticorps dgos28, anticorps GOSR1, anticorps gosr1, anticorps zgc:112064, anticorps GOLIM2, anticorps GOS-28, anticorps GOS28/P28, anticorps GS28, anticorps P28, anticorps AI414660, anticorps AI426320, anticorps BB145494, anticorps GOSRI, anticorps p28, anticorps Gos28, anticorps v-SNARE, anticorps Golgi SNARE, 28 kDa, anticorps golgi SNAP receptor complex member 1, anticorps Golgi SNAP receptor complex member 1, anticorps Gos28, anticorps GOSR1, anticorps gosr1, anticorps CpipJ_CPIJ014329, anticorps PAAG_02888, anticorps Tsp_07481, anticorps Gosr1, anticorps gos-28

Sujet: Most vesicular fusion events require the function of N-ethylmaleimide-sensitive factor (NSF) and the soluble NSF attachment proteins (SNAPs). Their function is dependent upon the SNAP receptor (SNARE) family of proteins. The specificity of vesicle docking and fusion is mediated by specific interactions between v-SNAREs on vesicles and t-SNAREs on target membranes. GS28 (Golgi SNARE with a size of 28 kDa) is a SNARE that associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi compartments. The majority of GS28, the first 230 and 250 aa, is thought to be anchored to the membrane via the C-terminal hydrophobic tail, which is formed by the remaining 20 aa. GS28 and syntaxin 5, another SNARE, exist as a protein complex in the Golgi and this complex is important for the function of both proteins in ER-Golgi transport. The GS28/syntaxin 5 complex can be dissociated by alpha-SNAP and NSF. In addition, GS28 is thought to interact with alpha-SNAP when the GS28/syntaxin 5 complex is dissociated. Thus, GS28 is a SNARE protein that mediates, in complex with syntaxin 5, transport within the Golgi and between Golgi and ER. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 28 kDa