KIF11 anticorps (AA 324-532)

N° du produit ABIN968407

Détail du produit anti-KIF11 anticorps

Antigène: KIF11 (Kinesin Family Member 11 (KIF11))

Épitope: AA 324-532

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIF11 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Eg5 aa. 324-532

Clone: 20-EG5

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-KIF11 (ABIN968407)

Ferhat, Cook, Chauviere, Harper, Kress, Lyons, Baas: "Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 19, pp. 7822-35, (1998) (PubMed).

Blangy, Lane, dHérin, Harper, Kress, Nigg: "Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo." dans: Cell, Vol. 83, Issue 7, pp. 1159-69, (1996) (PubMed).

Détails sur KIF11

Antigène: KIF11 (Kinesin Family Member 11 (KIF11))

Autre désignation: Eg5 (KIF11 Produits)

Synonymes: anticorps EG5, anticorps HKSP, anticorps KNSL1, anticorps MCLMR, anticorps TRIP5, anticorps Eg5, anticorps Kif8, anticorps Kifl1, anticorps Knsl1, anticorps F2H17.19, anticorps F2H17_19, anticorps DDBDRAFT_0189194, anticorps DDBDRAFT_0201556, anticorps DDB_0189194, anticorps DDB_0201556, anticorps XLEg5K1, anticorps XlEg5, anticorps cos2, anticorps costal2, anticorps eg5, anticorps hksp, anticorps kif11, anticorps kif11-a, anticorps kif11-b, anticorps kif11b, anticorps knsl1, anticorps trip5, anticorps Cos2, anticorps Costal2, anticorps XLEg5K2, anticorps kif11a, anticorps kinesin family member 11, anticorps P-loop containing nucleoside triphosphate hydrolases superfamily protein, anticorps kinesin family member 11 S homeolog, anticorps kinesin family member 11 L homeolog, anticorps KIF11, anticorps Kif11, anticorps AT2G36200, anticorps kif11, anticorps kif11.S, anticorps kif11.L

Sujet: The mitotic spindle apparatus equally distributes duplicated chromosomes to daughter cells. This process is mediated by the dynamic instability of the spindle microtubules and the forces generated by the action of cytoplasmic dynein and kinesin-related motor proteins (KRPs), such as CHO1/MKLP1. These motors hydrolyze ATP as they move progressively along the microtubules. They organize the microtubules into bipolar spindles and may also serve as bridges between microtubules and chromosome kinetochores or centrosomes. Eg5, identified in Xenopus laevis, is a plus-end directed KRP, associated with the mitotic spindle. Vertebrate Eg5 is a member of the KRP subfamily bimC. These family members share a high degree of sequence homology (>80% within the N-terminal motor domain) and may execute similiar functions. During mitosis, Eg5 is specifically phosphorylated at Thr-297, an evolutionarily conserved cdc2 phosphorylation site, by p34[cdc2]/cyclin B. Inhibition of phosphorylation blocks the interaction of Eg5 with centrosomes. Thus, Eg5 is a mitotic motor protein that regulates spindle formation in a phosphorylation-dependent manner. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 120 kDa