CD40 anticorps (AA 71-187)

N° du produit ABIN968495

Détail du produit anti-CD40 anticorps

Antigène: CD40

Épitope: AA 71-187

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human CD40 aa. 71-187

Clone: 41-CD40

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968584, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-CD40 (ABIN968495)

Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." dans: Immunity, Vol. 11, Issue 3, pp. 339-48, (1999) (PubMed).

Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." dans: Immunity, Vol. 11, Issue 3, pp. 379-89, (1999) (PubMed).

Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." dans: Immunity, Vol. 8, Issue 6, pp. 733-42, (1998) (PubMed).

Détails sur CD40

Antigène: CD40

Autre désignation: CD40 (CD40 Produits)

Synonymes: anticorps Bp50, anticorps CDW40, anticorps TNFRSF5, anticorps p50, anticorps AI326936, anticorps GP39, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TRAP, anticorps Tnfrsf5, anticorps TNFSF5, anticorps CD40 molecule, anticorps CD40 antigen, anticorps CD40, anticorps Cd40

Sujet: CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.

Poids moléculaire: 44 kDa

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints