AKAP4 anticorps (AA 555-675)

N° du produit ABIN968592

Détail du produit anti-AKAP4 anticorps

Antigène: AKAP4 (A Kinase (PRKA) Anchor Protein 4 (AKAP4))

Épitope: AA 555-675

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKAP4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse AKAP82 aa. 555-675

Clone: 25-AKAP82

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-AKAP4 (ABIN968592)

Edwards, Scott: "A-kinase anchoring proteins: protein kinase A and beyond." dans: Current opinion in cell biology, Vol. 12, Issue 2, pp. 217-21, (2000) (PubMed).

Miki, Eddy: "Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1." dans: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 34384-90, (1999) (PubMed).

Turner, Johnson, Haig-Ladewig, Gerton, Moss: "An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail." dans: The Journal of biological chemistry, Vol. 273, Issue 48, pp. 32135-41, (1998) (PubMed).

Fulcher, Mori, Welch, OBrien, Klapper, Eddy: "Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component." dans: Biology of reproduction, Vol. 52, Issue 1, pp. 41-9, (1995) (PubMed).

Détails sur AKAP4

Antigène: AKAP4 (A Kinase (PRKA) Anchor Protein 4 (AKAP4))

Autre désignation: AKAP82 (AKAP4 Produits)

Synonymes: anticorps AKAP4, anticorps AKAP 82, anticorps AKAP-4, anticorps AKAP82, anticorps CT99, anticorps FSC1, anticorps HI, anticorps PRKA4, anticorps hAKAP82, anticorps p82, anticorps Fsc1, anticorps A-kinase anchoring protein 4, anticorps A kinase (PRKA) anchor protein 4, anticorps AKAP4, anticorps Akap4

Sujet: The cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain the localization of PKA, the regulatory subunits interact with specific anchoring proteins. Several proteins have been identified as PKA anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Fifteen of the AKAP proteins contain a consensus binding motif that allows interaction with the type II regulatory subunit (RII) of the PKA holoenzyme. In addition, three other AKAPs (D-AKAP1, D-AKAP2, and fsc1/AKAP82) can associate with the type I regulatory subunit (RI) of the PKA holoenzyme. AKAP82 was isolated as a component of the mouse sperm fibrous sheath. It is a dual specificity AKAP that contains an RII-binding domain (domain A, amino acids 219 to 232) and an RI-binding domain (domain B, amino acids 335-344). In mouse, pro-AKAP82 is synthesized as a 97 kDa precursor that is transported to the flagellum where proteolytic cleavage of the N-terminal 179 amino acids produces AKAP82. Assembly of AKAP82 into the fibrous sheath surrounding the axoneme of the sperm flagellum is thought to tether PKA close to the axoneme where it can regulate flagellar motility.

Poids moléculaire: 82 kDa