HIP1R anticorps (AA 560-772)

N° du produit ABIN968746

Détail du produit anti-HIP1R anticorps

Antigène: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Épitope: AA 560-772

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HIP1R est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Humain, Chien, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Hip1R aa. 560-772

Clone: 44-Hip1R

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-HIP1R (ABIN968746)

Itoh, Koshiba, Kigawa, Kikuchi, Yokoyama, Takenawa: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis." dans: Science (New York, N.Y.), Vol. 291, Issue 5506, pp. 1047-51, (2001) (PubMed).

Engqvist-Goldstein, Kessels, Chopra, Hayden, Drubin: "An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles." dans: The Journal of cell biology, Vol. 147, Issue 7, pp. 1503-18, (2000) (PubMed).

Seki, Muramatsu, Sugano, Suzuki, Nakagawara, Ohhira, Hayashi, Hori, Saito: "Cloning, expression analysis, and chromosomal localization of HIP1R, an isolog of huntingtin interacting protein (HIP1)." dans: Journal of human genetics, Vol. 43, Issue 4, pp. 268-71, (1999) (PubMed).

Détails sur HIP1R

Antigène: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Autre désignation: Hip1R (HIP1R Produits)

Synonymes: anticorps HIP12, anticorps HIP3, anticorps ILWEQ, anticorps AA410023, anticorps mKIAA0655, anticorps HIP1R, anticorps hip1r, anticorps hip3, anticorps hip12, anticorps ilweq, anticorps DKFZp469N2015, anticorps hip1, anticorps MGC78813, anticorps huntingtin interacting protein 1 related, anticorps huntingtin interacting protein 1 related b, anticorps huntingtin interacting protein 1 related L homeolog, anticorps HIP1R, anticorps Hip1r, anticorps hip1rb, anticorps hip1r, anticorps hip1r.L

Sujet: Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192kDa each) and three light chains (23-25kDa each). A variety of proteins facilitate receptor-mediated endocytosis through association with clathrin-coated vesicles. Huntingtin interacting protein 1 (Hip1) is an actin-binding protein that interacts with Huntingtin protein, and has been implicated in vesicular transport defects found in Huntingtin's disease. Hip1 related protein (Hip1R) is another actin binding protein that contains an epsin NH2-terminal homology (ENTH)domain, three coiled-coil regions, a leucine zipper, and a talin-like actin binding domain. Hip1R mRNA is widely expressed, and Hip1R protein is enriched in the cell cortex and perinuclear region. The ENTH domain of Hip1R is required for binding to phosphatidylinositol-4,5-bisphosphate, and this complex is essential for clathrin-mediated endocytosis. In addition, Hip1R colocalizes with clathrin, AP-2 and endocytosed transferrin. Thus, Hip1R may facilitate interactions between clathrin-coated pits and actin during endocytosis.

Poids moléculaire: 120 kDa