Interleukin enhancer-binding factor 3 (ILF3) (AA 592-695) anticorps

N° du produit ABIN968762

Détail du produit

Antigène: Interleukin enhancer-binding factor 3 (ILF3)

Épitope: AA 592-695

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human DRBP76 aa. 592-695

Clone: 21-DRBP76

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Référence

Saunders, Jurecic, Barber: "The 90- and 110-kDa human NFAR proteins are translated from two differentially spliced mRNAs encoded on chromosome 19p13." dans: Genomics, Vol. 71, Issue 2, pp. 256-9, (2001) (PubMed).

Xu, Grabowski: "Molecular cloning and characterization of a translational inhibitory protein that binds to coding sequences of human acid beta-glucosidase and other mRNAs." dans: Molecular genetics and metabolism, Vol. 68, Issue 4, pp. 441-54, (2000) (PubMed).

Buaas, Lee, Edelhoff, Disteche, Braun: "Cloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteins." dans: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 10, Issue 5, pp. 451-6, (1999) (PubMed).

Patel, Vestal, Xu, Bandyopadhyay, Guo, Erme, Williams, Sen: "DRBP76, a double-stranded RNA-binding nuclear protein, is phosphorylated by the interferon-induced protein kinase, PKR." dans: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20432-7, (1999) (PubMed).

Détail du antigène

Antigène: Interleukin enhancer-binding factor 3 (ILF3)

Autre désignation: DRBP76 (ILF3 Produits)

Synonymes: anticorps CBTF, anticorps DRBF, anticorps DRBP76, anticorps MMP4, anticorps MPHOSPH4, anticorps MPP4, anticorps NF-AT-90, anticorps NF110, anticorps NF110b, anticorps NF90, anticorps NF90a, anticorps NF90b, anticorps NFAR, anticorps NFAR-1, anticorps NFAR2, anticorps TCP110, anticorps TCP80, anticorps MBII-26, anticorps ilf3, anticorps wu:fb37d07, anticorps wu:fb94b02, anticorps zgc:77030, anticorps 4F.1, anticorps cbtf122, anticorps ilf3-A, anticorps ubp3, anticorps ubp4, anticorps xilf3, anticorps interleukin enhancer binding factor 3, anticorps interleukin enhancer binding factor 3b, anticorps interleukin enhancer binding factor 3 S homeolog, anticorps ILF3, anticorps Ilf3, anticorps ilf3b, anticorps ilf3.S

Sujet: The double stranded RNA (dsRNA)-dependent, Ser/Thr protein kinase, PKR, is encoded by an IFN-inducible gene and is critical for the anti-viral responses mediated by IFN. Interaction with activators such as heparin, dsRNA, and the dsRNA-binding proteins (DRBPs), DRBP76, PACT, and RAX, induces PKR autophosphorylation and activation. DRBP76 was identified through its binding to dsRNA and PKR. In addition, DRBP76 has been identified as the alternatively spliced nuclear phosphoproteins of 90 kDa (NFAR-1/NF90) and 110 kDa (NFAR-2), as well as M-phase phosphoprotein (MPP4), translational control protein 80 (TCP80), and interleukin enhancer binding factor 3 (ILF3). DRBP76 contains a bi-partite nuclear localization signal at amino acids 369-373 and 386-394, two DRB domains in the C-terminal half, and a C-terminal RG2 domain that is present in many RNA binding proteins. DRBP76 binds pre-mRNAs and spliced mRNAs, co-localizes with PKR in the nucleus, and is phosphorylated by PKR and possibly cyclin-dependent kinases. Thus, DRBP76 may be one of multiple DRBP variants, which associate with PKR and regulate RNA translation. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 90 kDa

Pathways: M Phase