BUB1B anticorps (AA 276-388)
-
- Antigène Voir toutes BUB1B Anticorps
- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
-
Épitope
- AA 276-388
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp BUB1B est non-conjugé
-
Application
- Western Blotting (WB), Immunofluorescence (IF)
- Attributs du produit
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Human BUBR1 aa. 276-388
- Clone
- 9-BUBR1
- Isotype
- IgG2a
- Top Product
- Discover our top product BUB1B Anticorps primaire
-
-
- Commentaires
-
Related Products: ABIN968535, ABIN967389
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store undiluted at -20° C.
-
-
p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." dans: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).
: "Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast." dans: Nature, Vol. 392, Issue 6673, pp. 303-6, (1998) (PubMed).
: "
-
p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." dans: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).
-
- Antigène
- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
- Autre désignation
- BUBR1 (BUB1B Produits)
- Synonymes
- anticorps BUB1beta, anticorps BUBR1, anticorps Bub1A, anticorps MAD3L, anticorps MVA1, anticorps SSK1, anticorps hBUBR1, anticorps AU045529, anticorps bub1b, anticorps BUB1, anticorps BUB1-like, anticorps BubR1, anticorps BubRI, anticorps CG14030, anticorps CG14030/Bub1, anticorps Dmel\\CG14030, anticorps bub1, anticorps BUB1 mitotic checkpoint serine/threonine kinase B, anticorps BUB1B, mitotic checkpoint serine/threonine kinase, anticorps BUB1B, mitotic checkpoint serine/threonine kinase L homeolog, anticorps Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, anticorps Bub1 kinase, anticorps BUB1B, anticorps Bub1b, anticorps bub1b.L, anticorps bub1b, anticorps Bub1
- Sujet
- Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately atttached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. BUBR1 is a bub-related kinase homologous to Bub1 and MAD3. BUBR1 contains a nuclear localization signal and two highly conserved domains CD1 and CD2. CD1 is in the N-terminal region and is invoved in kinetochore localization, while CD2 is in the C-terminal region and is involved in kinase activity. BUBR1 is hyperphosphorylated during spindle checkpoint activation, and interacts with p55CDC and APC during spindle checkpoint function. In colorectal cancers, the BUBR1 gene has structural abnormalities that suggest BUBR1 is a tumor suppressor gene. Thus, BUBR1 may be a critical spindle checkpoint kinase and disruption of its function may be related to tumor formation.
- Poids moléculaire
- 125 kDa
-