SPTBN1 anticorps (AA 2101-2189)

N° du produit ABIN968882

Détail du produit anti-SPTBN1 anticorps

Antigène: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Épitope: AA 2101-2189

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SPTBN1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human beta-Spectrin II aa. 2101-2189

Clone: 42-B

Isotype: IgG1

Information d'application

Indications d'application: For western blot analysis use at 1:1000.

Commentaires:

Related Products: ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SPTBN1 (ABIN968882)

Nicolas, Fournier, Galand, Malbert-Colas, Bournier, Kroviarski, Bourgeois, Camonis, Dhermy, Grandchamp, Lecomte: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." dans: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, (2002) (PubMed).

Hu, Watanabe, Bennett: "Characterization of human brain cDNA encoding the general isoform of beta-spectrin." dans: The Journal of biological chemistry, Vol. 267, Issue 26, pp. 18715-22, (1992) (PubMed).

Moon, McMahon: "Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin." dans: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4427-33, (1990) (PubMed).

Détails sur SPTBN1

Antigène: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Autre désignation: beta-Spectrin II (SPTBN1 Produits)

Synonymes: anticorps 9930031C03Rik, anticorps AL033301, anticorps SPTB2, anticorps Spnb-2, anticorps Spnb2, anticorps elf1, anticorps elf3, anticorps mKIAA4049, anticorps ELF, anticorps betaSpII, anticorps Spnb1, anticorps spectrin beta, non-erythrocytic 1, anticorps spectrin, beta, non-erythrocytic 1, anticorps Sptbn1, anticorps SPTBN1

Sujet: Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, alpha and ß, which intertwine to form heterodimers that can self associate into elongated tetramers. alpha-spectin I and ß-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of alpha-spectin I and II with ß-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats alongwith various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. alpha-spectrin II is a widely expressed non-erythroid alpha-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. ß-spectrin II is a widely expressed non-erythroid ß-spectrin that contains a C-terminal region that interacts with alpha-spectrins and a PH domain. alpha-spectrin II and ß-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure. This antibody is routinely tested by the Western blot analysis.

Poids moléculaire: 280 kDa

Pathways: Regulation of Actin Filament Polymerization, Protein targeting to Nucleus