NEDD8 anticorps

N° du produit ABIN969547

Détail du produit anti-NEDD8 anticorps

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEDD8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Fonction: NEDD8 Antibody

Purification: Purified antibody

Immunogène: Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Clone: 5B8

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-NEDD8 (ABIN969547)

Ohki, Funatsu, Konishi, Chiba: "The mechanism of poly-NEDD8 chain formation in vitro." dans: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 443-7, (2009) (PubMed).

Détails sur NEDD8

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Autre désignation: NEDD8 (NEDD8 Produits)

Sujet:

Description: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Aliases: NEDD-8

Poids moléculaire: 9kDa

ID gène: 4738

HGNC: 4738

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway