DNASE1 anticorps

N° du produit ABIN98343

Détail du produit anti-DNASE1 anticorps

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNASE1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Deoxyribonuclease A from other tissues and species may occur but have not been specifically determined.

Pureté: Anti-DEOXYRIBONUCLEASE I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Deoxyribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-DEOXYRIBONUCLEASE I has been assayed against 1.0 µg of Deoxyribonuclease A [Bovine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1.500 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur DNASE1

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Autre désignation: Deoxyribonuclease I (DNASE1 Produits)

Synonymes: anticorps LOC397802, anticorps DNL1, anticorps DRNI, anticorps AI788650, anticorps DNaseI, anticorps Dnl1, anticorps dnase1-A, anticorps zgc:92440, anticorps DNase I, anticorps deoxyribonuclease 1, anticorps deoxyribonuclease I, anticorps deoxyribonuclease I L homeolog, anticorps Deoxyribonuclease I, anticorps endA, anticorps LOC397802, anticorps DNASE1, anticorps Dnase1, anticorps dnase1.L, anticorps dnase1, anticorps CpipJ_CPIJ011184, anticorps CpipJ_CPIJ011185, anticorps CpipJ_CPIJ011186, anticorps CpipJ_CPIJ011187, anticorps CpipJ_CPIJ011190, anticorps CpipJ_CPIJ017803, anticorps Desal_1409, anticorps MPET_RS05255, anticorps METEV_RS02275, anticorps Plabr_0631

Sujet: Synonyms: Deoxyribonuclease-1 EC=3.1.21.1 Deoxyribonuclease I DNase I

ID gène: 282217

UniProt: P00639