Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Réactivité croisée (Details)
Cross reactivity against Folate Binding Protein from other sources is unknown.
Pureté
Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].
niveau d'endotoxine
Low Endotoxin : No
Immunogène
Folate Binding Protein [Bovine Milk] Immunogen Type: Native Protein
FOLR1
Reactivité: Humain, Rat, Souris, Cheval, Lapin, Chien, Roussette (Chauve-souris), Hamster
WB, IHC, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Anti-Folate Binding Protein has been assayed against 1.0 µg of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Conseil sur la manipulation
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Date de péremption
Expiration date is one (1) year from date of opening.
Kameyama, Motoyama, Tanaka, Yamashita, Higashi, Arima: "Induction of mitophagy-mediated antitumor activity with folate-appended methyl-β-cyclodextrin." dans: International journal of nanomedicine, Vol. 12, pp. 3433-3446, (2017) (PubMed).
Anti-Folate Binding Protein antibody belongs to the folate receptor family and binds to folate and reduced folic acid derivatives. It further regulates delivery of 5-methyltetrahydrofolate to the cell interior. Folate expresses solely in epithelial tissues, and expression is increased in malignant tissues. Anti-Folate Binding Protein antibody is ideal for researchers investigating Tags & Cell Markers and Cancer research. Synonyms: Folate receptor alpha FR-alpha Folate receptor 1 Milk folate-binding protein Folate-binding protein 1 FBP