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FACS Antibodies

Le tri cellulaire activé par fluorescence (FACS) est un type spécialisé de cytométrie de flux. Il permet de trier un mélange hétérogène de cellules biologiques dans deux conteneurs ou plus, une cellule à la fois, sur la base des caractéristiques spécifiques de diffusion de la lumière et de fluorescence de chaque cellule et des anticorps qui y sont fixés.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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80.292 Résultats :

Cyclin D1 anticorps (AA 61-110)

CCND1 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Lapin, Mouton WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody at 1:5000 90min in 37˚C.

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (ABIN671016) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, unconjugated (ABIN671016) at 1: 200, followed by incubation with conjugated secondary antibody and DAB staining

RSC96 cells probed with Cyclin D1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 références

N° du produit ABIN671016

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FGF2 anticorps (AA 143-250)

FGF2 Reactivité: Humain, Souris, Rat, Lapin, Mouton WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

(a) Representative Masson staining images of the heart papillary muscle cross-sectional scan images, and infarct rate of the different groups.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated (ABIN726425) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-BFGF/FGF2 Polyclonal Antibody, Unconjugated (ABIN726425) followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cells probed with Rabbit Anti-bFGF Polyclonal Antibody

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse spleen lysates probed with bFGF Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

HepG2 lysates probed with bFGF Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

(a) Representative images and bar graphs of the transmigrated cell number in the labeled and unlabeled endothelial progenitor cell (EPC) groups (x200).

9 images

18 références

N° du produit ABIN726425

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NF-kB p65 anticorps (pSer536)

NFkBP65 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet WB, ELISA, IHC (p), FACS Hôte: Lapin Polyclonal unconjugated

SeMet reversed activation of TLR4/NF-κB pathway induced by ESBL-E.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (ABIN730528) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Necrostatin-1 attenuated neuroinflammatory changes in a rat neuropathic pain model.

SeMet inhibited activation of TLR4/NF-κB pathway induced by ESBL-E.

8 images

12 références

N° du produit ABIN730528

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CXCR4 anticorps (AA 201-294)

CXCR4 Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Mouse lung lysates probed with Anti-CXCR4 Polyclonal Antibody at 1:5000 for 90 min at 37˚C.

L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184/CXCR4 Polyclonal Antibody, Unconjugated (ABIN730888) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (ABIN730888), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human U937 cells probed withCXCR4 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (ABIN730888), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-CXCR4 Polyclonal Antibody .

8 images

16 références

N° du produit ABIN730888

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NLRP3 anticorps (AA 15-120)

NLRP3 Reactivité: Humain, Souris, Rat, Chien WB, ELISA, FACS, IHC (p), IF (p), ICC, IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HeLa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

15 images

11 références

N° du produit ABIN1386361

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MMP2 anticorps (AA 31-109)

MMP2 Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin embedded rat carotid artery Anti-MMP-2 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.

HT1080 lysates probed with MMP-2 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

HeLa cells probed with MMP2 Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Paraformaldehyde-fixed, paraffin embedded Rat Brain Antigen retrieval by boiling in sodium citrate buffer (pH6.

6 images

17 références

N° du produit ABIN668286

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Cytochrome C anticorps (AA 51-105)

CYCS Reactivité: Humain, Rat, Souris, Lapin, Poulet WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (ABIN723590) 1:300 followed by conjugation to the secondary antibody and DAB staining

L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (ABIN723590) at 1:200 overnight at 4 °C.

Mouse heart probed with Anti-Cytochrome C Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

Image kindly submitted by an end-user via Funakoshi.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathwayAfter transfection with Ad-Srx-1, cells were then exposure to SI/R.

Srx-1 enhanced cardiomyocyte resistance to SI/R injury by the PI3K/AKT signalling(A) Western blotting analysis for phospho-AKT and AKT in H9c2 cell treated with Ad-Srx-1 upon SI/R.

Analysis of activation of the intrinsic apoptotic pathway.

9 images

19 références

N° du produit ABIN723590

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Interferon gamma anticorps (AA 75-155)

IFNG Reactivité: Souris, Rat WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Diseased mouse lung and spleen lysates probed with Anti-IFN gamma Polyclonal Antibody at 1:5000 90min in 37˚C.

Antigen: 0.

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated (ABIN669126) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mice were anesthetized and then infected intranasally by dropping 0.

Mice were anesthetized and then infected intranasally by dropping 0.

7 images

20 références

N° du produit ABIN669126

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IL-6 anticorps (AA 111-150)

IL6 Reactivité: Humain, Souris, Rat, Lapin, Boeuf (Vache) WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200, followed by conjugation to the secondary antibody and DAB staining

L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

14 références

N° du produit ABIN728083

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CD31 anticorps (AA 601-680)

PECAM1 Reactivité: Humain, Souris, Rat, Lapin, Porc, Chien FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Recombinant human CD31 protein probed with CD31 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed: Endothelial cells labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated at 1:200 for 40 minutes at 37°

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Rat spleen cells probed with Rabbit Anti-CD31 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

8 images

15 références

N° du produit ABIN669006

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alpha Tubulin anticorps (FITC)

TUBA1 Reactivité: Humain, Souris, Porc, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Dindon, Paramecium ICC, FACS Hôte: Souris Monoclonal TU-01 FITC

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/mL, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/mL, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Nucleus is stained with DAPI (blue).

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red).

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red).

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

8 images

11 références

N° du produit ABIN93892

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alpha Tubulin anticorps (Biotin)

TUBA1 Reactivité: Humain, Souris, Porc, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Dindon, Paramecium WB, IHC (p), ICC, FACS Hôte: Souris Monoclonal TU-01 Biotin

Western blotting analysis of human alpha-tubulin using mouse monoclonal antibody TU-01 on lysates of various cell lines under reducing and non-reducing conditions.

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red).

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red).

Nucleus is stained with DAPI (blue).

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Western blotting analysis of alpha-tubulin in porcine brain lysate by antibody TU-01 biotin.

7 images

10 références

N° du produit ABIN301997

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TUBB3 anticorps (N-Term) (FITC)

TUBB3 Reactivité: Humain, Souris, Rat, Chien, Porc FACS, ICC Hôte: Souris Monoclonal TU-20 FITC

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml).

Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μL reagent per million cells in 100 μL of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/mL, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml).

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/mL).

6 images

10 références

N° du produit ABIN93912

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beta Actin anticorps

ACTB Reactivité: Humain, Souris, Rat WB, IF, IHC (p), FACS, DNA Mic Hôte: Souris Monoclonal 137CT26-1-1 unconjugated

All lanes : Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded H.

Confocal immunofluorescent analysis of ACTB Antibody (ABIN658990 and ABIN2838035) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of anti-ACTB Antibody (ABIN658990 and ABIN2838035) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

(ABIN658990 and ABIN2838035) staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

7 images

12 références

N° du produit ABIN658990

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FcRn anticorps

FcRn Reactivité: Humain ELISA, IF, FACS, cELISA, Func Hôte: Souris Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity.

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE.

Tracer plasma concentrations were determined by ELISA and plotted either as percent remaining as compared with the first time point plasma concentrations or as plasma concentrations ±SD.

ELISA binding of human, macaque, pig, dog, mouse, and rat FcRn toward ADM31 at pH 7.

Identification of monoclonal Abs that block albumin binding to hFcRn.

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone ADM31 primary Ab and Texas Red™ conjugated anti-mouse secondary.

8 images

15 références

N° du produit ABIN1774762

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