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FACS Antibodies

Le tri cellulaire activé par fluorescence (FACS) est un type spécialisé de cytométrie de flux. Il permet de trier un mélange hétérogène de cellules biologiques dans deux conteneurs ou plus, une cellule à la fois, sur la base des caractéristiques spécifiques de diffusion de la lumière et de fluorescence de chaque cellule et des anticorps qui y sont fixés.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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80.648 Résultats :

FcRn anticorps

FcRn Reactivité: Humain ELISA, IF, FACS, cELISA, Func Hôte: Souris Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity.

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE.

Tracer plasma concentrations were determined by ELISA and plotted either as percent remaining as compared with the first time point plasma concentrations or as plasma concentrations ±SD.

ELISA binding of human, macaque, pig, dog, mouse, and rat FcRn toward ADM31 at pH 7.

Identification of monoclonal Abs that block albumin binding to hFcRn.

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone ADM31 primary Ab and Texas Red™ conjugated anti-mouse secondary.

8 images

15 références

N° du produit ABIN1774762

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Liver Arginase anticorps

KO Validated ARG1 Reactivité: Humain WB, ELISA, FACS, IHC (p), IF, IP, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE antibody diluted at 1:50000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I , antibody at 1:200 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I , antibody at 1:500 dilution.

WB Image Arginase 1 antibody detects Arginase 1 protein by western blot analysis.

Elevated right ventricular pressure, increased right ventricular weight and altered expression of Ddah1 and Arg1 in cavin-/- mice.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Arg-1 expression in neutrophils and lymph-node microenvironmentNeutrophils isolated from control and HL patients were analysed for Arg-1 expression (A), activity by measuring urea (B) and protein expression by Western Blot (C).

8 images

11 références

N° du produit ABIN2856661

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SQSTM1 anticorps

KO Validated SQSTM1 Reactivité: Humain WB, IF, IP, IHC (p), FACS, ICC, PLA Hôte: Lapin Polyclonal unconjugated

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.

IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at 1:1000

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot analysis.

IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal, Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometry analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

HDAC2 positively regulates the expression of p-Akt, p-mTOR, and survivin in ER+ breast cancer cells.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Celastrol activates autophagy and inhibits MPP+-induced neurotoxicity in dopaminergic neurons.

Celastrol enhances the clearance of damaged mitochondria via activating mitophagy.

23 images

13 références

N° du produit ABIN2854836

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CD25 anticorps (AA 201-268)

IL2RA Reactivité: Humain, Souris FACS, WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Lane 1: EC9706 lysates Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL2RA Polyclonal Antibody, Unconjugated (ABIN670371) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

Independent Validation

1 validation

N° du produit ABIN670371

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PALB2 anticorps (AA 1101-1186)

PALB2 Reactivité: Humain, Porc WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1101-1186) antibody (ABIN670536)

Images provided by the Independent Validation Program (badge number 29749).

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (ABIN670536) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

4 images

Independent Validation

1 validation

N° du produit ABIN670536

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MEK2 anticorps (AA 1-50)

MAP2K2 Reactivité: Humain, Souris WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)

Images provided by the Independent Validation Program, badge number 029748.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250.

4 images

Independent Validation

1 validation

N° du produit ABIN726500

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FOXO1 anticorps (AA 165-270)

FOXO1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poulet WB, ELISA, IF (p), IHC (p), FACS Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (ABIN1387620) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

4 images

Independent Validation

1 validation

N° du produit ABIN1387620

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BAD anticorps (AA 101-204)

BAD Reactivité: Humain, Souris, Rat ELISA, WB, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 101-204) antibody (ABIN674709)

Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Polyclonal Antibody, Unconjugated (ABIN674709) at 1:300 overnight at 4 °C.

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29802).

4 images

Independent Validation

1 validation

N° du produit ABIN674709

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ALX4 anticorps (AA 249-275)

ALX4 Reactivité: Humain WB, FACS, CUT&RUN Hôte: Lapin Polyclonal RB28072 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) western blot analysis in MDA-M cell line lysates (35 μg/lane).

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) flow cytometric analysis of MDA-M cells (right histogram) compared to a negative control cell (left histogram).

4 images

Independent Validation

1 validation

N° du produit ABIN654344

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BrdU anticorps

BrdU FACS, IHC (p), ICC, IHC (fro) Hôte: Souris Monoclonal Bu20a unconjugated

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Flow Cytometry (FACS) image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

3 images

1 reference

Independent Validation

2 validations

N° du produit ABIN2477713

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Integrin beta 2 anticorps

ITGB2 Reactivité: Humain FACS, WB, IP, IHC (fro), IHC (p) Hôte: Souris Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 images

5 références

N° du produit ABIN94005

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ICAM1 anticorps

ICAM1 Reactivité: Humain, Rat, Boeuf (Vache) WB, FACS, ELISA, IHC (p), ICC Hôte: Souris Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions.

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111.

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

6 images

7 références

N° du produit ABIN94177

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Transferrin Receptor anticorps

TFRC Reactivité: Humain FACS, IP, ICC Hôte: Souris Monoclonal MEM-75 unconjugated

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE .

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green).

Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75, green).

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

6 images

1 reference

N° du produit ABIN94221

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pan Keratin anticorps

panKRT Reactivité: Mammifères FACS, WB, IHC (p), ICC, IP Hôte: Souris Monoclonal C-11 unconjugated

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 ((ABIN94275), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green).

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

6 images

6 références

N° du produit ABIN94275

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Cytokeratin 18 anticorps

KRT18 Reactivité: Humain WB, FACS, ELISA, IHC (p), IP, ICC Hôte: Souris Monoclonal DC-10 unconjugated

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Detectionof cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10 .

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 ((ABIN94282), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

6 images

4 références

N° du produit ABIN94282

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