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FACS Antibodies

Le tri cellulaire activé par fluorescence (FACS) est un type spécialisé de cytométrie de flux. Il permet de trier un mélange hétérogène de cellules biologiques dans deux conteneurs ou plus, une cellule à la fois, sur la base des caractéristiques spécifiques de diffusion de la lumière et de fluorescence de chaque cellule et des anticorps qui y sont fixés.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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80.292 Résultats :

ICAM1 anticorps

ICAM1 Reactivité: Humain, Rat, Boeuf (Vache) WB, FACS, ELISA, IHC (p), ICC Hôte: Souris Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions.

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111.

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

6 images

7 références

N° du produit ABIN94177

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Cytokeratin 19 anticorps

KRT19 Reactivité: Humain WB, ELISA, FACS, IHC (p), IP, ICC Hôte: Souris Monoclonal A53-B-A2 unconjugated

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (ABIN94290, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions.

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

6 images

2 références

N° du produit ABIN94290

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CD63 anticorps

CD63 Reactivité: Humain FACS, IHC (p), IP, ICC Hôte: Souris Monoclonal MEM-259 unconjugated

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259, green) after co-incubation of living cells with human Transferrin - Dyomics 547 (cat.

Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259, green).

Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259, green).

9 images

3 références

N° du produit ABIN94214

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Cytokeratin 18 anticorps

KRT18 Reactivité: Mammifères WB, FACS, ELISA, IHC (p), IP, ICC Hôte: Souris Monoclonal C-04 unconjugated

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) Fig.

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 () FITC.

Flow cytometry intracellular staining pattern of HeLa cell suspension stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/ml) GAM FITC.

Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/mL, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

8 images

4 références

N° du produit ABIN94279

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ZAP70 anticorps (C-Term)

ZAP70 Reactivité: Humain WB, FACS, ICC Hôte: Souris Monoclonal ZAP-03 unconjugated

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 ; 0.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western blotting analysis of human ZAP70 using mouse monoclonal antibody ZAP-03 on lysates of Jurkat cell line and HEK-293T cell line (negative control) under reducing and non-reducing conditions.

6 images

1 reference

N° du produit ABIN125756

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Vimentin anticorps

VIM Reactivité: Mammifères WB, FACS, IHC (p), ICC Hôte: Souris Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunofluorescence staining (rat basophils)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

9 images

4 références

N° du produit ABIN94491

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SIT1 anticorps

SIT1 Reactivité: Humain WB, IP, FACS Hôte: Souris Monoclonal SIT-01 unconjugated

Western blot of human Jurkat T cell line

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions.

Anti-SIT Purified (clone SIT-01) works in WB application.

Anti-SIT Purified (clone SIT-01) specificity verification by WB.

7 images

2 références

N° du produit ABIN94465

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TUBG1 anticorps (C-Term)

TUBG1 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poulet, Plantes, Protozoa, Porc WB, ICC, FACS Hôte: Souris Monoclonal TU-30 unconjugated

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions.

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Fig.

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (; direct conjugate with Dyomics 547, red).

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red).

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts.

8 images

8 références

N° du produit ABIN94313

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LAT2 anticorps (Cytoplasmic Domain)

LAT2 Reactivité: Humain, Souris WB, IP, ICC, FACS Hôte: Souris Monoclonal NAP-07 unconjugated

Western blotting analysis of human NTAL using mouse monoclonal antibody NAP-07 on lysates of Raji and Ramos cells and on Jurkat cells (negative control) under reducing and non-reducing conditions.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Confocal microscopy Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.

Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.

Separation of human CD3 negative NTAL positive lymphocytes (red-filled) from CD3 positive NTAL negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

6 images

5 références

N° du produit ABIN94439

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CD63 anticorps (Biotin)

CD63 Reactivité: Humain FACS, IHC (p), IP, ICC Hôte: Souris Monoclonal MEM-259 Biotin

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

7 images

3 références

N° du produit ABIN1027697

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Growth Hormone Receptor anticorps (AA 101-200)

GHR Reactivité: Humain, Souris, Rat, Porc, Mouton WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Evaluation of the pig GHR transcript with a 4bp insert in vitro.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (ABIN671481) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated .

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Knockout of the pig GHR gene in F2 homozygous pigs (GHR4bp/4bp refers to -/-).

6 images

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N° du produit ABIN671481

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HMGB1 anticorps (AA 75-170)

HMGB1 Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated .

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

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N° du produit ABIN671616

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beta Catenin anticorps (pSer33, pSer37)

CATNB Reactivité: Humain, Souris, Rat WB, FACS, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon cancer tissue labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

7 images

1 reference

N° du produit ABIN683893

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CD28 anticorps (AA 1-120)

CD28 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded Human stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

1 reference

N° du produit ABIN674604

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CCR7 anticorps (AA 25-59)

CCR7 Reactivité: Humain, Souris, Rat, Mouton FACS, WB, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (ABIN674724) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (ABIN674724) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy5 conjugated used at 1:200 dilution

Formalin-fixed and paraffin embedded human tubular adenoma labeled with Anti-CCR7 CD197 Polyclonal Antibody, unconjugated (ABIN674724) followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Raji probed with CCR7/CD197 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

6 images

3 références

N° du produit ABIN674724

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