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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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251 Résultats :

PI3K p85 alpha/gamma anticorps (pTyr199, pTyr467)

PI3K p85a/g Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 références

Independent Validation

1 validation

N° du produit ABIN744743

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PD-1 anticorps (AA 201-288)

PDCD1 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-D-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 overnight at 4 °C.

5 images

2 références

Independent Validation

1 validation

N° du produit ABIN735608

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AKT 1/2/3 anticorps (AA 401-479)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

5 images

9 références

Independent Validation

1 validation

N° du produit ABIN725195

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EIF4E anticorps (AA 121-217)

EIF4E Reactivité: Humain, Rat WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (ABIN1386772) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Independently Validated Antibody, image provided by Science Direct, badge number 029640:Formalin-fixed and paraffin embedded human pancreas labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (ABIN1386772) at 1:250 followed by conjugation to the secondary antibody and DAB staining

7 images

Independent Validation

1 validation

N° du produit ABIN1386772

Fiche technique

Fiche de données

CD200R1L anticorps (AA 151-250)

CD200R1L Reactivité: Humain WB, ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C.

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Staining of Placental villus from 7w gestation karyotype-normal pregnancy with Antibody.

5 images

4 références

Independent Validation

2 validations

N° du produit ABIN1715098

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Fiche de données

TNFSF9 anticorps (AA 101-200)

TNFSF9 Reactivité: Humain, Souris, Rat WB, ELISA, ICC, IF (p), IF (cc), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Independently Validated Antibody, image provided by Science Direct, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

9 images

Independent Validation

1 validation

N° du produit ABIN705056

Fiche technique

Fiche de données

IDE anticorps (AA 491-590)

IDE Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody (ABIN723680)

Western Blotting validation image for anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody (ABIN723680)

Formalin-fixed and paraffin-embedded human gastric carcinoma labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated (ABIN723680) at 1:300 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse tumor labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated (ABIN723680) at 1:300 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-IDE Polyclonal Antibody, Unconjugated (ABIN723680) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-IDE Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse tumor labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded Mouse Brain Antigen retrieval by boiling in sodium citrate buffer (pH6.

8 images

Independent Validation

2 validations

N° du produit ABIN723680

Fiche technique

Fiche de données

MEK1 anticorps (AA 2-150)

MAP2K1 Reactivité: Humain ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Image provided by the Independent Validation Program (badge number 29760).

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (ABIN686482) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

Independent Validation

1 validation

N° du produit ABIN686482

Fiche technique

Fiche de données

NFKB1 anticorps (AA 860-969)

NFKB1 Reactivité: Humain WB, ELISA, IF, IHC (p), PLA Hôte: Souris Monoclonal 4A11 unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody (ABIN561981)

Western Blot detection against Immunogen (37.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon.

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

8 images

Independent Validation

1 validation

N° du produit ABIN561981

Fiche technique

Fiche de données

CREB1 anticorps

CREB1 Reactivité: Humain WB, IHC, ELISA, ICC Hôte: Souris Monoclonal 5G3 unconjugated

Western Blotting validation image for anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061)

Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.

Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng),

Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green).

5 images

3 références

Independent Validation

1 validation

N° du produit ABIN969061

Fiche technique

Fiche de données

NeuN anticorps (AA 1-140)

RBFOX3 Reactivité: Humain WB, ELISA, ICC, FACS Hôte: Souris Monoclonal 1H3B2 unconjugated

Western Blotting validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Immunohistochemistry validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Western blot analysis using RBFOX3 mAb against human RBFOX3 (AA: 1-140) recombinant protein.

Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green).

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.

Flow cytometric analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).

8 images

Independent Validation

2 validations

N° du produit ABIN5542704

Fiche technique

Fiche de données

SYN1 anticorps (AA 362-511)

SYN1 Reactivité: Humain WB, ELISA, IHC, ICC, FACS Hôte: Souris Monoclonal 7H10G6 unconjugated

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

Western blot analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein.

Immunohistochemical analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.

Western blot analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.

Flow cytometric analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of GC-7901 cells.

Immunofluorescence analysis of GC-7901 cells using SYN1 mouse mAb (green).

Immunofluorescence analysis of HepG2 cells.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunofluorescence analysis of HepG2 cells using SYN1 mouse mAb (green).

12 images

Independent Validation

1 validation

N° du produit ABIN5542390

Fiche technique

Fiche de données

TLR9 anticorps (AA 868-1016)

TLR9 Reactivité: Humain IHC, ELISA, FACS, ICC Hôte: Souris Monoclonal 1B12H2 unconjugated

Immunofluorescence validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Proximity Ligation Assay validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein.

Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TLR9 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TLR9 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using TLR9 mAb against HEK293 (1) and TLR9 (AA: 868-1016)-hIgGFc transfected HEK293 (2) cell lysate.

9 images

Independent Validation

2 validations

N° du produit ABIN5542492

Fiche technique

Fiche de données

APP anticorps (C-Term)

APP Reactivité: Humain WB, IF, IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody (ABIN2855011)

WB Image Sample(30 μg of whole cell lysate) A:Hep G2, 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP, antibody(10 μg/ml).

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm and nucleus by immunofluorescent analysis.

IHC-P Image APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.

WB Image Various whole cell extracts (30 μg) were separated by 7.

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm by confocal immunofluorescent analysis.

8 images

Independent Validation

1 validation

N° du produit ABIN2855011

Fiche technique

Fiche de données

ARPC2 anticorps

ARPC2 Reactivité: Humain WB, ICC, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2, antibody at 1:100 dilution.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2, antibody at 1:200 dilution.

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

WB Image Sample(30 μg of whole cell lysate) A:293T B: HeLa S3, 12% SDS PAGE antibody diluted at 1:500

7 images

Independent Validation

1 validation

N° du produit ABIN2855249

Fiche technique

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