Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CDJ1 anticorps (N-Term)

CDJ1 Reactivité: Chlamydomonas reinhardtii IP, WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN334548
  • Antigène
    CDJ1 (Chloroplast DnaJ Protein (CDJ1))
    Épitope
    N-Term
    Reactivité
    Chlamydomonas reinhardtii
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Réactivité croisée (Details)
    Not reactive in: higher plants
    Homologie
    Ostreococcus sp.
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa
    Purification
    serum
    Immunogène
    fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
  • Indications d'application
    1. 3000 with standard ECL (WB)
    Commentaires

    western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." dans: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

    Willmund, Dorn, Schulz-Raffelt, Schroda: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." dans: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).

  • Antigène
    CDJ1 (Chloroplast DnaJ Protein (CDJ1))
    Autre désignation
    CDJ1
    Synonymes
    anticorps chloroplast DnaJ-like protein, anticorps CDJ1
    Sujet
    CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
    Poids moléculaire
    expected: 40 kDa, apparent: 40 kDa
    UniProt
    Q66YD3
Vous êtes ici:
Support technique