CLEC4A2 Kit ELISA
CLEC4A2
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951053
-
-
Antigène
-
CLEC4A2
(C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
-
Reactivité
-
Rat
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Rat CLEC4A2 ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Indications d'application
-
Optimal working dilution should be determined by the investigator.
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Date de péremption
-
6 months
-
-
Antigène
-
CLEC4A2
(C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
-
Autre désignation
-
CLEC4A2
-
Synonymes
-
Clec4a Kit ELISA, Clecsf6 Kit ELISA, DCIR Kit ELISA, Dcir1 Kit ELISA, Dcir2 Kit ELISA, C-type lectin domain family 4, member a2 Kit ELISA, C-type lectin domain family 4, member A2 Kit ELISA, Clec4a2 Kit ELISA
-
ID gène
-
297584
-
UniProt
-
Q5YIS1
-