Custom Polyclonal Antibodies
Having delivered high-quality polyclonal antibodies to biopharma, diagnostics, and academic scientists for 40+ years, our partner Rockland Immunochemicals, Inc. (Rockland) has the experience and wide-ranging expertise to efficiently drive your custom polyclonal antibody production project to a successful completion.
Every polyclonal antibody production project is customized to your specific needs and can cover any or all of the workflow steps—consultation, peptide or protein antigen production, antibody generation, purification, and conjugation of reporter molecules—with antibodies produced in a range of species, including New Zealand white rabbits, goats, sheep, guinea pigs, chickens, rats, mice, and donkeys.
Our Custom Polyclonal Antibody Production Process
Each project is performed in distinct phases—and you decide if and when to initiate each phase.
Phase 1: Antigen Preparation
We can use a range of macromolecules as antigens, as long as they can be used under BSL-2 conditions, and can design and produce custom peptides and recombinant proteins.
- Protein, recombinant or purified
- Synthetic peptide Modified peptide
- Haptens
- Whole cell lysate
- Nucleic acid
- Lipids
- Carbohydrate complexes
- And more (i.e. viruses, bacteria, toxins, etc.)
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Our Service Partner: Rockland
Our Service Partner: Rockland
Benefit from expertise over two generations of scientific advances. Rockland enabling biomolecule detection, characterization and analysis since 1962. 60,000 square feet manufacturing facility produce high-quality antibodies, reagents and custom services for your next project
Benefit from expertise over two generations of scientific advances. Rockland enabling biomolecule detection, characterization and analysis since 1962. 60,000 square feet manufacturing facility produce high-quality antibodies, reagents and custom services for your next project
Phase 2: Polyclonal Antibody Development
During polyclonal antibody development, we immunize based on the schedule you request and can recommend an adjuvant to achieve your project goals. We provide a pre-bleed serum (5 mL) for screening in your labs and can also use our experience and monitoring of the antibody-mediated immune response to make recommendations prior to the collection of production bleeds.
Upon conclusion of the applied protocol, you are presented with the option of collecting a terminal bleed (this only applies to rabbits) or by extending your study for the collection of additional bleeds on a monthly basis.
Phase 3: Antibody Purification
After antibody development, we can use a number of different methods to purify your polyclonal antibodies.
- Protein A purification
- Protein G purification
- Protein A/G purification
- Affinity purification
- IgG purification
- IgY purification
- And more (i.e. BMP, gold, latex beads, etc.)
Phase 4: Antibody Conjugation
We provide a wide array of customizable conjugation services to meet specific assay and reagent needs.
Conjugates include the following:
- Biotin
- Horseradish peroxidase (HRP)
- Alkaline peroxidase Fluorochrome/dyes
Our Antigen Specialization
We are experts in generating antibodies specific for post-translational modifications— including phospho-epitopes, acetylation, methylation, hydroxylation, ubiquitinylation, and more—using modified peptides, and can optimize peptide design and synthesis as well as recombinant protein design and preparation.
