This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
Attributs du produit
Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
Purification
Protein A chromatography
Pureté
> 90 % pure
Immunogène
Purified protein derivative (PPD)
Clone
BDI578
Isotype
IgG2a
Indications d'application
ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0,1 mg/mL (OD280nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
Heat Shock Protein 65 (HSP65)
Autre désignation
Heat Shock Protein 65 / HSP65
Synonymes
anticorps CPN60, anticorps GROEL, anticorps HLD4, anticorps HSP-60, anticorps HSP60, anticorps HSP65, anticorps HuCHA60, anticorps SPG13, anticorps heat shock protein family D (Hsp60) member 1, anticorps HSPD1
Sujet
Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65