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Souris IgM Isotype Control

IsoC, FACS IgM unconjugated
N° du produit ABIN2145425
  • Antigène Tous les produits IgM
    IgM
    Hôte
    • 57
    • 37
    • 37
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Souris
    Application
    Isotype Control (IsoC), Flow Cytometry (FACS)
    Attributs du produit
    This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
    Purification
    Purified
    Isotype
    IgM
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Procédure de l'essai
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
    • Repeat step 5.
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4,
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Antigène
    IgM
    Abstract
    IgM Produits
    Synonymes
    IgM constant region Isotype Control, IGM Isotype Control
    Classe de substances
    Antibody
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