Souris IgG1 Isotype Control
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- Antigène Tous les produits IgG1
- IgG1
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Hôte
- Souris
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Clonalité
- Monoclonal
- Application
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
- Aucune reactivité croisée
- Humain, Rat
- Purification
- Purified by protein-A affinity chromatography.
- Pureté
- > 95 % (by SDS-PAGE)
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Clone
- MOPC-21
- Isotype
- IgG1 kappa
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- Indications d'application
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Do not freeze.
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Do not freeze.
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Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." dans: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).
: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." dans: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).
: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." dans: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).
: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." dans: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).
: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." dans: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, (2005) (PubMed).
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Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." dans: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).
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- Antigène
- IgG1
- Abstract
- IgG1 Produits
- Synonymes
- IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control
- Classe de substances
- Antibody
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