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Llama IgG3 Isotype Control

FACS, ELISA, WB IgG3 unconjugated
N° du produit ABIN5608800
  • Antigène Tous les produits IgG3
    IgG3
    Reactivité
    Llama
    Hôte
    • 54
    • 7
    • 5
    • 1
    • 1
    Llama
    Application
    Flow Cytometry (FACS), ELISA, Western Blotting (WB)
    Purification
    Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to llama IgG1 or IgG2 are observed.
    Isotype
    IgG3
  • Indications d'application

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG3 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Commentaires

    Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    IgG3
    Abstract
    IgG3 Produits
    Synonymes
    IgG3 Isotype Control, Immunoglobulin heavy constant gamma 3 Isotype Control, immunoglobulin heavy constant gamma 3 (G3m marker) Isotype Control, Ighg3 Isotype Control, IGHG3 Isotype Control
    Classe de substances
    Antibody
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