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Llama IgG3 Isotype Control

Name: Llama IgG3 Isotype Control
Brand: antibodies-online
SKU: ABIN5608800
Price: 256.3 EUR
Availability: InStock

FACS, ELISA, WB IgG3 unconjugated

SDS-PAGE of Llama IgG3 Isotype Control. (Llama IgG3 Isotype Control)

SDS

1 image

N° du produit ABIN5608800

Fiche de données

Antigène Tous les produits IgG3

IgG3

Reactivité

Llama
Hôte
54

7

5

1

1
Llama

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB)

Purification

Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to llama IgG1 or IgG2 are observed.

Isotype

IgG3
Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE

Mouse IgG3 isotype control (FITC)
FACS, ELISA Monoclonal B10 IgG3 FITC

Mouse IgG3 isotype control (SPRD)
FACS, ELISA Monoclonal B10 IgG3 SPRD

Mouse IgG3 isotype control (APC)
FACS, ELISA Monoclonal B10 IgG3 APC

Mouse IgG3 isotype control (Biotin)
FACS, ELISA Monoclonal B10 IgG3 Biotin

Mouse IgG3 isotype control (PE-Cy7)
FACS, ELISA Monoclonal B10 IgG3 PE-Cy7

Mouse IgG3 isotype control
FACS, ELISA Monoclonal B10 IgG3 unconjugated

Mouse IgG3 isotype control
FACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated

Mouse IgG3 isotype control (FITC)
FACS, ELISA, IsoC, WB, FM Monoclonal MG3 IgG3 FITC

Human IgG3 isotype control
IsoC Polyclonal IgG3 unconjugated

Indications d'application

Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG3 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized

Commentaires

Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption

12 months
Antigène

IgG3

Abstract

IgG3 Produits

Synonymes

IgG3 Isotype Control, Immunoglobulin heavy constant gamma 3 Isotype Control, immunoglobulin heavy constant gamma 3 (G3m marker) Isotype Control, Ighg3 Isotype Control, IGHG3 Isotype Control

Classe de substances

Antibody