Souris IgG2a isotype control (APC)
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- Antigène Tous les produits IgG2a
- IgG2a
- Reactivité
- Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
- Conjugué
- APC
- Application
- Flow Cytometry (FACS), ELISA, FLISA, Fluorescence Microscopy (FM)
- Purification
- Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2b , IgG3 , IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
- Isotype
- IgG2a
- Labeling Ratio
- 1-2
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- Indications d'application
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2a isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000-1:20,000
IF Microscopy Dilution: User Optimized
- Commentaires
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Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 3 and μ - IgM, light chain κ and λ.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Stabilizer: None
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
- Date de péremption
- 12 months
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- Antigène
- IgG2a
- Abstract
- IgG2a Produits
- Synonymes
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Classe de substances
- Antibody
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