Cathepsin D Kit ELISA

N° du produit ABIN1112571

Détail du produit Cathepsin D Kit ELISA

Antigène: Cathepsin D (CTSD)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: For quantitative detection of Cathepsin D in human serum, plasma or cell culture supernatants.

Type d'échantillon: Serum, Plasma, Cell Culture Supernatant

Analytical Method: Quantitative

Sensibilité: < 10 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-human Cathepsin D antibody 2. Lyophilized human Cathepsin D standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human Cathepsin D antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cathepsin D polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cathepsin D polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Cathepsin D amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Cathepsin D can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 15 min at 1500 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: Cathepsin D (CTSD)

Autre désignation: Cathepsin D (CTSD Produits)

Synonymes: catD Kit ELISA, fb93e11 Kit ELISA, fj17b09 Kit ELISA, wu:fb93e11 Kit ELISA, wu:fj17b09 Kit ELISA, LOC398557 Kit ELISA, Cathd Kit ELISA, CatD Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, DKFZp469J0315 Kit ELISA, DDBDRAFT_0205785 Kit ELISA, DDBDRAFT_0215012 Kit ELISA, DDB_0205785 Kit ELISA, DDB_0215012 Kit ELISA, CLN10 Kit ELISA, CPSD Kit ELISA, CD Kit ELISA, cathepsin D Kit ELISA, cathepsin D S homeolog Kit ELISA, lysosomal aspartic protease Kit ELISA, cathepsin D-like Kit ELISA, ctsd Kit ELISA, LOC398557 Kit ELISA, ctsd.S Kit ELISA, CatD Kit ELISA, LOC5567565 Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, Cathd Kit ELISA, ctsD Kit ELISA, catd Kit ELISA, Ctsd Kit ELISA, LOC443060 Kit ELISA

Sujet: Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. It is an aspartic protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes where it degrades insulin. Mutations in the CTSD gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. It also has been used as a breast cancer tumor marker.

Pathways: Peptide Hormone Metabolism