CD147 Kit ELISA

N° du produit ABIN1112595

Détail du produit CD147 Kit ELISA

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: For quantitative detection of Emmprin in tissue lysates or cell culture supernates.

Type d'échantillon: Tissue Lysate, Cell Culture Supernatant

Analytical Method: Quantitative

Sensibilité: < 2 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Human Emmprin antibody 2. Lyophilized Human Emmprin standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Emmprin antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human- Emmprin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Emmprin polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Emmprin amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Emmprin can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernate, tissue lysate or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge at 2-8°C for 15 min at 1000 x g within 30 min of collection. For eliminating the platelet effect, suggesting that further centrifugation for 10 min at 2-8°C at 10000 x g. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: Emmprin / CD147 (BSG Produits)

Synonymes: EMMPRIN Kit ELISA, RPE7 Kit ELISA, 5F7 Kit ELISA, CD147 Kit ELISA, M6 Kit ELISA, OK Kit ELISA, TCSF Kit ELISA, AI115436 Kit ELISA, AI325119 Kit ELISA, HT-7 Kit ELISA, 5A11 Kit ELISA, CE9 Kit ELISA, HT7 Kit ELISA, Ox47R Kit ELISA, basignin Kit ELISA, wu:fb26a05 Kit ELISA, wu:fo98e12 Kit ELISA, bsg Kit ELISA, prso03 Kit ELISA, basigin (Ok blood group) Kit ELISA, basigin Kit ELISA, basigin (Ok blood group) S homeolog Kit ELISA, BSG Kit ELISA, Bsg Kit ELISA, bsg Kit ELISA, bsg.S Kit ELISA

Sujet: Cluster of Differentiation 147 (CD147) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin (BSG), is a member of the immunoglobulin superfamily, It interacts with fibroblasts and stimulates expression of MMPs, and plays an important role in tumor invasiveness and metastasis. This protein is a determinant for the Ok blood group system, and it has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum.

Pathways: S100 Proteins