Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CX3CL1 Kit ELISA

CX3CL1 Reactivité: Souris Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
N° du produit ABIN1112608
  • Antigène Voir toutes CX3CL1 Kits ELISA
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    Reactivité
    • 10
    • 9
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    For quantitative detection of Fractalkine in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Sensibilité
    < 10 pg/mL
    Ingrédients
    1. One 96-well plate pre-coated with anti-mouse fractalkine antibody 2. Lyophilized mouse fractalkine standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-mouse fractalkine antibody (Concentrated): 130 µl.
    Matériel non inclus
    1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
    Featured
    Discover our best selling CX3CL1 Kit ELISA
    Top Product
    Discover our top product CX3CL1 Kit ELISA
  • Commentaires

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-fractalkine polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-fractalkine polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the fractalkine amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of fractalkine can be calculated.

    Plaque
    Pre-coated
    Préparation des réactifs
    1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
    Préparation de l'échantillon

    Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
    Tissue lysate, body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
    Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
    Plasma: Collect plasma with heparin as the anticoagulant. Centrifuge for 20 min at 2000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate and EDTA can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

    Restrictions
    For Research Use only
  • Agent conservateur
    Sodium azide, Thimerosal (Merthiolate)
  • Antigène Voir toutes CX3CL1 Kits ELISA
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    Autre désignation
    Fractalkine / CX3CL1 (CX3CL1 Produits)
    Synonymes
    ABCD3 Kit ELISA, CX3CL1 Kit ELISA, DKFZp459K117 Kit ELISA, Cx3c Kit ELISA, Scyd1 Kit ELISA, ABCD-3 Kit ELISA, C3Xkine Kit ELISA, CXC3 Kit ELISA, CXC3C Kit ELISA, NTN Kit ELISA, NTT Kit ELISA, SCYD1 Kit ELISA, fractalkine Kit ELISA, neurotactin Kit ELISA, AB030188 Kit ELISA, AI848747 Kit ELISA, CX3C Kit ELISA, Cxc3 Kit ELISA, D8Bwg0439e Kit ELISA, ATP binding cassette subfamily D member 3 Kit ELISA, C-X3-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X3-C motif) ligand 1 Kit ELISA, ABCD3 Kit ELISA, CX3CL1 Kit ELISA, Cx3cl1 Kit ELISA, Abcd3 Kit ELISA
    Sujet
    Chemokine (C-X3-C motif) ligand 1 (CX3CL1), also known as fractalkine (in humans) and neurotactin (in mice), is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.
    Pathways
    Synaptic Membrane
Vous êtes ici:
Support technique