Prostate Specific Antigen Kit ELISA

N° du produit ABIN1112732

Détail du produit Prostate Specific Antigen Kit ELISA

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Analytical Method: Quantitative

Sensibilité: < 10 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Human Kallikrein 3 antibody 2. Lyophilized Human Kallikrein 3 standards: 2 tubes (20ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Kallikrein 3 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Kallikrein 3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Kallikrein 3 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Kallikrein 3 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Kallikrein 3 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: Kallikrein 3 / PSA (PSA Produits)

Synonymes: APS Kit ELISA, KLK2A1 Kit ELISA, PSA Kit ELISA, hK3 Kit ELISA, KLK3 Kit ELISA, Klk1c10l2 Kit ELISA, RSGK-50 Kit ELISA, RSKG-50 Kit ELISA, rGK-3 Kit ELISA, Kal-3 Kit ELISA, Kal3 Kit ELISA, Klk3 Kit ELISA, AAP-S Kit ELISA, MP100 Kit ELISA, Psa Kit ELISA, R74825 Kit ELISA, goku Kit ELISA, kallikrein related peptidase 3 Kit ELISA, kallikrein 3 Kit ELISA, kallikrein 1-related peptidase C3 Kit ELISA, kallikrein B, plasma 1 Kit ELISA, aminopeptidase puromycin sensitive Kit ELISA, KLK3 Kit ELISA, Klk1c3 Kit ELISA, Klkb1 Kit ELISA, Npepps Kit ELISA

Sujet: Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a single-chain glycoprotein with a molecular mass of about 33 kD that may function normally in the liquefaction of seminal coagulum. The PSA gene spans about 6 kb and contains 5 exons. It is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is produced for the ejaculate, where it liquefies semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving cervical mucus, allowing the entry of sperm into the uterus.

Pathways: Système du Complément