Immunoglobulin isotyping

N° du produit ABIN1113186

Détail du produit

Antigène: Ig

Épitope: Heavy & Light Chain

Reactivité: Souris

Méthode de détection: Colorimetric

Conjugué: HRP

Application: Isotyping (IsoT)

Fonction: The method employs the quantitative sandwich enzyme immunoassay technique.

Marque: FastELISA®

Type d'échantillon: Cell Culture Supernatant, Purified Protein

Specificité: Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,

Attributs du produit: The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.

Information d'application

Commentaires:

One Step ELISA 30 min total time

Durée du test: < 1 h

Plaque: Pre-coated

Protocole: Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Préparation des réactifs:

dilution

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Ig

Autre désignation: Immunoglobulins (Ig Produits)

Synonymes: ATPIG Kit, ATPIQ Kit, A330005H02Rik Kit, AI315324 Kit, Ig Kit, ATPase phospholipid transporting 11C Kit, ATPase, class VI, type 11C Kit, ATP11C Kit, Atp11c Kit

Sujet: Isotyping of mouse immunoglobulins