IgG Kit ELISA
-
- Antigène Voir toutes IgG Kits ELISA
- IgG
- Épitope
- Heavy & Light Chain
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 20-1900 ng/mL
- Seuil minimal de détection
- 20 ng/mL
- Application
- ELISA
- Fonction
- Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process
- Marque
- FastELISA®
- Type d'échantillon
- Cell Culture Supernatant, Ascitic Fluid
- Analytical Method
- Quantitative
- Specificité
- The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).
- Réactivité croisée (Details)
- < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.
- Sensibilité
- 6 ng/mL
- Attributs du produit
- The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
- Featured
- Discover our best selling IgG Kit ELISA
- Top Product
- Discover our top product IgG Kit ELISA
-
-
- Commentaires
-
One Step ELISA 30 min total time
- Durée du test
- < 1 h
- Plaque
- Pre-coated
- Protocole
- A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Préparation des réactifs
-
dilution
- Restrictions
- For Research Use only
-
- Stock
- 4 °C
- Date de péremption
- 12 months
-
- Antigène Voir toutes IgG Kits ELISA
- IgG
- Autre désignation
- Immunoglobulin G (IgG Produits)
- Classe de substances
- Antibody
-