PPIA Kit ELISA
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- Antigène Voir toutes PPIA Kits ELISA
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.25 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.25 pg/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYPA in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of this index.
- Réactivité croisée (Details)
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- Sensibilité
- 12.8 pg/mL
- Ingrédients
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- Matériel non inclus
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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- Volume d'échantillon
- 100 μL
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - Procédure de l'essai
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- Conseil sur la manipulation
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- Date de péremption
- 12 months
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- Antigène Voir toutes PPIA Kits ELISA
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
- Autre désignation
- CYPA (PPIA Produits)
- Synonymes
- Cyp1 Kit ELISA, PPIA-2 Kit ELISA, fa93g09 Kit ELISA, ppia Kit ELISA, wu:fa93g09 Kit ELISA, wu:fb05e11 Kit ELISA, wu:fb13h02 Kit ELISA, wu:fb15a05 Kit ELISA, CYPA Kit ELISA, CypA Kit ELISA, Cypa Kit ELISA, CYPH Kit ELISA, CYCA Kit ELISA, CyP-A Kit ELISA, MGC75715 Kit ELISA, 2700098C05 Kit ELISA, Cphn Kit ELISA, CyP-18 Kit ELISA, MT-ND1 Kit ELISA, MTND1 Kit ELISA, NADH1 Kit ELISA, ND1 Kit ELISA, cypA Kit ELISA, PPIase A Kit ELISA, ppial Kit ELISA, wu:fj18g05 Kit ELISA, zgc:73102 Kit ELISA, zgc:86688 Kit ELISA, peptidylprolyl isomerase A Kit ELISA, peptidylprolyl isomerase Aa (cyclophilin A) Kit ELISA, peptidylprolyl isomerase A (cyclophilin A) L homeolog Kit ELISA, cyclophilin A Kit ELISA, cyclophilin a Kit ELISA, peptidylprolyl isomerase A (cyclophilin A) Kit ELISA, peptidylprolyl isomerase Ab (cyclophilin A) Kit ELISA, PPIA Kit ELISA, ppiaa Kit ELISA, ppia.L Kit ELISA, Cypa Kit ELISA, CNB01230 Kit ELISA, CNB01290 Kit ELISA, Tc00.1047053506925.300 Kit ELISA, Tb11.03.0250 Kit ELISA, CYPA Kit ELISA, Ppia Kit ELISA, ppia Kit ELISA, ppiab Kit ELISA
- Sujet
- Alternative name: PPIA, CyP-A, CYPH, Peptidylprolyl Isomerase A, Peptidyl-Prolyl Isomerase A, Peptidyl-prolyl cis-trans isomerase A, Cyclosporin A-binding protein
- ID gène
- 25518
- UniProt
- P10111
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